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anti-ATPase, H+ Transporting, Lysosomal V0 Subunit D2 (ATP6V0D2) Antibodies

Subunit of the integral membrane V0 complex of vacuolar ATPase.

list all antibodies Gene Name GeneID UniProt
Anti-Mouse ATP6V0D2 ATP6V0D2 242341 Q80SY3
ATP6V0D2    
ATP6V0D2    
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Top anti-ATP6V0D2 Antibodies at antibodies-online.com

Showing 7 out of 7 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Arabidopsis thaliana Rabbit Un-conjugated IHC, WB Western Blot analysis of samples from yeast whole cell extract expressing the Arabidopsis thaliana subunit E1. Primary antibodies have been used at 1: 1: 000. Secondary antibodies at 1: 3 000. The load per well was 10 ug of total protein. Samples were separated on 10 % gel in Tris glycine SDS buffer. The proteins were transferred to a membrane (Schleicher & Schuell), blocked with 5% dry milk in Tris-buffered saline before incubation for 2 hours up to over night at RT with the primary antibody. A series of washes with TTBS was followed by incubation with a secondary antibody from). Western Blotting: 1 - molecular weight markers (Precision Plus Protein Standards, Dual Color, Bio-Rad, 10ul), 2 -Arabidopsis thaliana leaf extract (25 ug) Experimental Conditions: Protein was separated on a 12% SDS-PAGE gel at 200 V for approximately 40 min using the mini-Protein 3 cell (Bio-Rad). Protein was transferred to PVDF at 100 V for 1h using the mini-TransBlot cell (Bio-Rad). Blocked overnight at 4ºC in 5% non-fat milk dissolved in 1xPBST. Incubated with anti-V-ATPase at 1:2 000 for 2h at RT. After several washes in 1xPBST, blot was incubated with goat anti-rabbit IgG-alkaline phosphatase conjugate (Bio-Rad) at 1:3000 for 1h at RT. Blot was developed with NBT/BCIP (Molecular Probes). Courtesy: Ms Jaimie Schnell, PhD candidate 50 μL Log in to see 5 to 6 Days
$375.38
Details
Arabidopsis thaliana Goat Un-conjugated WB 300 μL Log in to see 5 to 6 Days
$467.78
Details
Arabidopsis thaliana Rabbit Un-conjugated ELISA, WB 1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-V-ATPase subunit A antibodies (ABIN349654, 1:2000,1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. 100 μL Log in to see 5 to 6 Days
$628.32
Details
Rice Rabbit Un-conjugated ELISA, WB 65ug/lane of purified vacuolar membranes from Vigna radiata L. (1,3) and purifiedV-ATPase, 7.4ug/lane (2,4) were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters wereblocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membraneswere washed 5 times with TBS-T, each time in a fresh polystyrene box and probedwith anti-V-ATPase subunit A antibodies (ABIN349685, 1:2000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. CBB - staining with Coomasie blue - left panel. Immunoblot - western blot detectoin -right panel. 100 μL Log in to see 5 to 6 Days
$628.32
Details
Arabidopsis thaliana Rabbit Un-conjugated ELISA, WB   100 μL Log in to see 5 to 6 Days
$628.32
Details
Arabidopsis thaliana Rabbit Un-conjugated ELISA, WB   100 μL Log in to see 5 to 6 Days
$628.32
Details
Arabidopsis thaliana Goat Un-conjugated WB   200 μg Log in to see 5 to 6 Days
$497.81
Details

ATP6V0D2 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality
Arabidopsis thaliana
,

Tomato (Solanum lycopersicum)
,

Top referenced anti-ATP6V0D2 Antibodies

  1. Arabidopsis thaliana Polyclonal ATP6V0D2 Primary Antibody for IHC, WB - ABIN3197488 : Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells. in The Journal of biological chemistry 2009 (PubMed)
    Show all 10 references for ABIN3197488

  2. Rice (Oryza sativa) Polyclonal ATP6V0D2 Primary Antibody for ELISA, WB - ABIN349685 : Kawamura, Arakawa, Maeshima, Yoshida: Tissue specificity of E subunit isoforms of plant vacuolar H(+)-ATPase and existence of isotype enzymes. in The Journal of biological chemistry 2000 (PubMed)
    Show all 4 references for ABIN349685

  3. Arabidopsis thaliana Polyclonal ATP6V0D2 Primary Antibody for ELISA, WB - ABIN349654 : Kobae, Mizutani, Segami, Maeshima: Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells. in Bioscience, biotechnology, and biochemistry 2006 (PubMed)

  4. Arabidopsis thaliana Polyclonal ATP6V0D2 Primary Antibody for WB - ABIN361273 : McLoughlin, Galvan-Ampudia, Julkowska, Caarls, van der Does, Laurière, Munnik, Haring, Testerink: The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress. in The Plant journal : for cell and molecular biology 2012 (PubMed)

More Antibodies against ATP6V0D2 Interaction Partners

Mouse (Murine) ATPase, H+ Transporting, Lysosomal V0 Subunit D2 (ATP6V0D2) interaction partners

  1. Insulin (show INS Antibodies) significantly activated ERK1/2 (show MAPK1/3 Antibodies) MAP kinase (show MAPK1 Antibodies) as well as markedly induced the expression of NFATc1 (show NFATC1 Antibodies), an osteoclast marker gene, and Atp6v0d2, an osteoclast fusion-related gene.

  2. V-ATPase (show ATP6V1H Antibodies) has a role in modulating a macrophage phenotype towards tumor-associated macrophages through the action of the a2 isoform of V-ATPase (show ATP6V1H Antibodies)

  3. Luteolin can be effective in reducing bone resorption and that this effect of luteolin may be through disruption of osteoclast V-ATPase a3 (show TCIRG1 Antibodies)-d2 interaction.

  4. PKCdelta (show PKCd Antibodies) deficiency does not perturb formation of the ruffled border or trafficking of lysosomal vesicles containing the vacuolar-ATPase (v-ATPase (show DNAH8 Antibodies)).

  5. these findings suggest that the critical role of ATP6v0d2 may be limited to the control of bone homeostasis under normal development.

  6. The data indicate that integrity of lipid rafts regulates the activity of V-ATPase (show ATP6V1H Antibodies) in osteoclasts, suggesting that cholesterol-lowering agents might be useful for inhibiting osteoclast-dependent bone resorption.

  7. Atp6v0d2 is a regulator of osteoclast fusion and bone formation.

  8. Data indicate for the first time that the NFATc1 (show NFATC1 Antibodies)/Atp6v0d2 and DC-STAMP (show TM7SF4 Antibodies) signaling axis plays a key role in the osteoclast multinucleation process, which is essential for efficient bone resorption.

  9. Atp6v0d2 (d2), an isoform of the d subunit in the V-ATPase (show ATP6V1H Antibodies), showed 5-fold higher expression than that of Atp6v0d1 (show ATP6V0D1 Antibodies) (d1) in mature osteoclasts, indicating a potential function in osteoclastic bone resorption.

  10. MEF2 (show MEF2C Antibodies) and MITF (show MITF Antibodies) function cooperatively with NFATc1 (show NFATC1 Antibodies) to transactivate the V-ATPase (show ATP6V1H Antibodies) d2 promoter during RANKL (show TNFSF11 Antibodies)-induced osteoclastogenesis.

ATP6V0D2 Antigen Profile

Protein Summary

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). May play a role in coupling of proton transport and ATP hydrolysis. Regulator of osteoclast fusion and bone formation.

Gene names and symbols associated with ATP6V0D2

  • ATPase, H+ transporting, lysosomal V0 subunit D2 (Atp6v0d2) antibody
  • vacuolar proton pump3 (vpp3) antibody
  • 1620401A02Rik antibody
  • AI324824 antibody
  • V-ATPase antibody

Protein level used designations for ATP6V0D2

ATPase, H+ transporting, V0 subunit D , V-ATPase subunit d 2 , V-type proton ATPase subunit d 2 , osteoclast-specific vacuolar ATP synthase , vacuolar proton pump subunit d 2 , vacuolar proton-translocating ATPase d subunit d2 , IDP1607 , IDP1607a , IDP1607c , IDP1662 , IDP2575 , IDP2575a , IDP2575c , PCO134411 , PCO134411(361) , PCO134411_ov , PZA00936 , V-ATPase , V-type H+-ATPase , nPZA00936-1 , vacuolar ATPase 69 kDa subunit , vpp*-U36436

GENE ID SPECIES
242341 Mus musculus
542327 Zea mays
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