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anti-Autophagy Related 12 (ATG12) Antibodies

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Additionally we are shipping ATG12 Proteins (19) and ATG12 Kits (7) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ATG12 9140 O94817
ATG12 67526 Q9CQY1
ATG12 361321 Q2TBJ5
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Top anti-ATG12 Antibodies at antibodies-online.com

Showing 10 out of 202 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IF, WB Western blot analysis of hAPG12L-T23 (ABIN387986) in MCF-7 cell line lysates (35 µg/lane). APG12L (arrow) was detected using the purified polyclonal antibody. Western blot analysis of hAPG12L-T23 (ABIN387986) in mouse brain tissue lysates (35 µg/lane). APG12L (arrow) was detected using the purified polyclonal antibody. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated IF, IHC (p), WB Western blot analysis of anti-APG12L Pab (ABIN388536) in Hela cell lysate. APG12L (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB Formalin-fixed and paraffin-embedded human cancer tissue reacted with Autophagy APG12L Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Mouse Un-conjugated IF, IHC (p), WB Western blot analysis of anti-APG12 Monoclonal Antibody (ABIN387794) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (ABIN387794), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Yeast Rabbit Un-conjugated EIA, WB Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of ROCKLAND's anti-GFP (code # 600-101-215) followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX (code # 605-703-125). Panel C shows specific reaction with APG12 using a 1:2,000 dilution of ROCKLAND's IgG fraction of Rabbit-anti-APG12 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX (code # 611-103-122). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin- 2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. 0.5 mg Log in to see 6 to 8 Days
$500.50
Details
Human Rabbit Un-conjugated IF (p), IHC (p) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti Apg12 Polyclonal Antibody,Unconjugated (ABIN752518) at 1:200 followed by conjugation to the secondary antibody and DAB staining. Formalin-fixed and paraffin embedded mouse Kidney tissue labeled with Anti Apg12 Polyclonal Antibody, unconjugated (ABIN752518) at 1:200 followed by incubation with conjugated secondary antibody and DAB staining. 100 μL Log in to see 3 to 7 Days
$269.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB Immunohistochemistry: ATG12 antibody staining of Human brain tissue at 2.5 μg/ml. Western blot. ATG12 antibody staining of Mouse heart tissue lysate at 1 µg/ml. A: Absence of Blocking peptide B: Presence of Blocking peptide 0.1 mg Log in to see 6 to 8 Days
$401.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB Immunohistochemistry: ATG12 antibody staining of Human brain tissue at 2.5 μg/ml. Western blot: ATG12 antibody staining of Human brain tissue lysate at (A) 0.5 and (B) 1 μg/ml. 0.1 mg Log in to see 6 to 8 Days
$401.50
Details
Human Rabbit Un-conjugated WB WB Suggested Anti-ATG12 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysate WB Suggested Anti-ATG12  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500  Positive Control:.21_B cell lysate  ATG12 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IHC, WB WB Suggested Anti-Atg12 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Rat Kidney 100 μL Log in to see 2 to 3 Days
$289.00
Details

ATG12 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-ATG12 Antibodies

  1. Human Polyclonal ATG12 Primary Antibody for IF, IHC (p) - ABIN388536 : Lum, DeBerardinis, Thompson: Autophagy in metazoans: cell survival in the land of plenty. in Nature reviews. Molecular cell biology 2005 (PubMed)
    Show all 8 references for ABIN388536

  2. Yeast (Saccharomyces cerevisiae) Polyclonal ATG12 Primary Antibody for EIA, WB - ABIN116953 : Krysko, Samoylenko, Polishchuk, Andronati, Kabanova, Khristova, Kuzmin, Kabanov, Krysko, Varnek, Grygorash: RGD mimetics containing phthalimidine fragment as novel ligands of fibrinogen receptor. in Bioorganic & medicinal chemistry letters 2011 (PubMed)
    Show all 8 references for ABIN116953

  3. Human Monoclonal ATG12 Primary Antibody for IF, IHC (p) - ABIN387794 : Xia, Zhang, Chen, Zhang, Liu, Jin, Ma, Ma, Yuan: Control of basal autophagy by calpain1 mediated cleavage of ATG5. in Autophagy 2010 (PubMed)
    Show all 6 references for ABIN387794

  4. Human Polyclonal ATG12 Primary Antibody for EIA, IHC (p) - ABIN497964 : Gozuacik, Kimchi: Autophagy as a cell death and tumor suppressor mechanism. in Oncogene 2004 (PubMed)
    Show all 5 references for ABIN497964

  5. Human Polyclonal ATG12 Primary Antibody for IF, WB - ABIN387986 : Tanida, Nishitani, Nemoto, Ueno, Kominami: Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing. in Biochemical and biophysical research communications 2002 (PubMed)
    Show all 5 references for ABIN387986

  6. Human Polyclonal ATG12 Primary Antibody for EIA, IHC (p) - ABIN497963 : Jounai, Takeshita, Kobiyama, Sawano, Miyawaki, Xin, Ishii, Kawai, Akira, Suzuki, Okuda: The Atg5 Atg12 conjugate associates with innate antiviral immune responses. in Proceedings of the National Academy of Sciences of the United States of America 2007 (PubMed)
    Show all 5 references for ABIN497963

  7. Human Polyclonal ATG12 Primary Antibody for EIA, IHC (p) - ABIN1449610 : Yee, Wilkinson, James, Ryan, Vousden: PUMA- and Bax-induced autophagy contributes to apoptosis. in Cell death and differentiation 2009 (PubMed)
    Show all 2 references for ABIN1449610

  8. Human Polyclonal ATG12 Primary Antibody for IF (p), IHC (p) - ABIN752518 : Song, Kou, Zou, Gao, Zeng, Xie: Involvement of autophagy in tri-ortho-cresyl phosphate- induced delayed neuropathy in hens. in Neurochemistry international 2013 (PubMed)

More Antibodies against ATG12 Interaction Partners

Human Autophagy Related 12 (ATG12) interaction partners

  1. Analysis of hepatitis B virus capsid maturation steps revealed that Rab33B and Atg5/12/16L1 are required for proper particle assembly and/or stability.

  2. reveal a novel interconnection between autophagy, proteasome activity, and cell death mediated by the ubiquitin-like properties of ATG12

  3. The results revealed that the expression of Atg12 and LC3 (show MAP1LC3A Antibodies) II/LC3 (show MAP1LC3A Antibodies) I in the chondrocytes treated with TXC increased, compared to that in the untreated chondrocytes

  4. Cav-1 competitively interacts with the ATG12-ATG5 system to suppress the formation and function of the latter in lung epithelial cells.

  5. ATG12 is a novel determinant of breast cancer primary resistance to HER1 (show EGFR Antibodies)/2-targeted therapeutics.

  6. study to identify role of conjugation between ATG12 and ATG5 in LC3 lipidation; structural and mutational analyses of ATG12~ATG5-ATG16N revealed the conjugation generates a patch across ATG12 and ATG5 required for E3 activity

  7. These data relate LC3B, ATG5 and ATG12 to mitochondrial quality control after oxidative damage, and to cellular longevity.

  8. The Atg12 is as a positive mediator of mitochondrial apoptosis and show that Atg12 directly regulates the apoptotic pathway by binding and inactivating prosurvival Bcl-2 (show BCL2 Antibodies) family members, including Bcl-2 (show BCL2 Antibodies) and Mcl-1 (show MCL1 Antibodies).

  9. Calpain1 plays an important role in controlling the levels of autophagy in normal living cells by regulating the levels of a key signaling molecule, ATG12-ATG5 conjugate.

  10. Human Apg3p (show ATG3 Antibodies)/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16 (show GABARAPL2 Antibodies), GABARAP (show GABARAP Antibodies), and MAP-LC3 (show MAP1LC3A Antibodies), and facilitates the conjugation of hApg12p to hApg5p

Mouse (Murine) Autophagy Related 12 (ATG12) interaction partners

  1. Although genetic deletion of either Atg12 or Atg5 (show ATG5 Antibodies) renders POMC (show POMC Antibodies) neurons autophagy-deficient, mice lacking Atg5 (show ATG5 Antibodies) in POMC (show POMC Antibodies) neurons do not exhibit these phenotypes.

  2. ATG12-ATG3 (show ATG3 Antibodies) interacts with Alix (show PDCD6IP Antibodies) to promote basal autophagic flux and late endosome function.

  3. Atg5 (show ATG5 Antibodies)-Atg12/Atg16L1 (show ATG16L1 Antibodies) protein complex is required for IFNgamma-mediated host defense against murine norovirus infection.

  4. Vaccinia virus actively disrupts the cellular autophagy through a novel molecular mechanism that is associated with aberrant LC3 (show MAP1LC3A Antibodies) lipidation and a direct conjugation between ATG12 and ATG3 (show ATG3 Antibodies).

  5. Despite forming the Atg12-Atg5-Atg16L2 complex, Atg16L2 is not recruited to phagophores and is mostly present in the cytosol.

  6. Autophagy participated the process of immune response of anti-MTB (show NCAPG2 Antibodies). Atg12 were the important molecule which control the formation of autophagy when MTB (show NCAPG2 Antibodies) infected.

  7. Overexpression of Atg5 or Atg12 resulted in Atg5-Atg12 conjugate formation and suppression of RNA-mediated signaling.

ATG12 Antigen Profile

Protein Summary

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998

Gene names and symbols associated with ATG12

  • ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12) antibody
  • autophagy related 12 (ATG12) antibody
  • autophagy related 12 (Atg12) antibody
  • similar to S. cerevisiae ATG12 (YBR217W) which nucleates preautophagosome formation as a conjugate with Atg5 (ATG12) antibody
  • 4931423H11Rik antibody
  • A330058M13Rik antibody
  • APG12 antibody
  • Apg12l antibody
  • Atg12l antibody
  • FBR93 antibody
  • HAPG12 antibody

Protein level used designations for ATG12

autophagy-related protein 12 , ubiquitin-like protein ATG12 , ATG12 autophagy related 12 homolog , Apg12 (autophagy, yeast) homolog , autophagy-related 12 , potential preautophagosome nucleating protein Atg12

GENE ID SPECIES
767903 Bos taurus
9140 Homo sapiens
67526 Mus musculus
361321 Rattus norvegicus
3636186 Candida albicans SC5314
Selected quality suppliers for anti-ATG12 (ATG12) Antibodies
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