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anti-BRF1 Homolog, Subunit of RNA Polymerase III Transcription Initiation Factor IIIB (S. Cerevisiae) (BRF1) Antibodies

BRF1 encodes one of the three subunits of the RNA polymerase III transcription factor complex. Additionally we are shipping BRF1 Proteins (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
BRF1 72308  
BRF1 2972 Q92994
BRF1 299347  
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Top anti-BRF1 Antibodies at antibodies-online.com

Showing 10 out of 68 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Mouse Rabbit Un-conjugated WB WB Suggested Anti-BRF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Thymus 100 μL Log in to see 2 to 3 Days
$289.00
Details
Fruit Fly Rabbit Un-conjugated WB WB Suggested Anti-BRF1 Antibody Titration: 1.25ug/mlELISA Titer: 1:62500Positive Control: NIH/3T3 cell lysate 100 μL Log in to see 2 to 3 Days
$229.00
Details
Human Rabbit Un-conjugated EIA, WB BRF1 Antibody (Center) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the BRF1 antibody detected the BRF1 protein (arrow). 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated WB BRF1 Antibody (Center) (ABIN658721) western blot analysis in Hela cell line lysates (35 µg/lane). This demonstrates the BRF1 antibody detected the BRF1 protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Mouse Rabbit Un-conjugated WB 100 μg Log in to see 8 to 10 Days
$493.17
Details
Human Rabbit Un-conjugated IF, ELISA, WB 100 μL Log in to see 8 to 10 Days
$478.50
Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of BRF1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of BRF1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 9 Days
$487.50
Details
Mouse Rabbit Un-conjugated ELISA, WB 50 μg Log in to see 2 to 3 Days
$433.13
Details
Human Mouse Un-conjugated DB, ICC Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BRF1 antibody. 50 μg Log in to see 7 to 9 Days
$285.71
Details
Mouse Rabbit Un-conjugated ELISA, WB 100 μg Log in to see 2 to 3 Days
$343.75
Details

BRF1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Mouse (Murine) , , ,



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Rat (Rattus) , , ,



Top referenced anti-BRF1 Antibodies

  1. Human Polyclonal BRF1 Primary Antibody for EIA, WB - ABIN950677 : Haurie, Durrieu-Gaillard, Dumay-Odelot, Da Silva, Rey, Prochazkova, Roeder, Besser, Teichmann: Two isoforms of human RNA polymerase III with specific functions in cell growth and transformation. in Proceedings of the National Academy of Sciences of the United States of America 2010 (PubMed)
    Show all 2 references for ABIN950677

  2. Mouse (Murine) Polyclonal BRF1 Primary Antibody for WB - ABIN2778626 : Raineri, Wegmueller, Gross, Certa, Moroni: Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference. in Nucleic acids research 2004 (PubMed)

  3. Fruit Fly (Drosophila melanogaster) Polyclonal BRF1 Primary Antibody for WB - ABIN2780068 : Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ... in Proceedings of the National Academy of Sciences of the United States of America 2002 (PubMed)

More Antibodies against BRF1 Interaction Partners

Fruit Fly (Drosophila melanogaster) BRF1 Homolog, Subunit of RNA Polymerase III Transcription Initiation Factor IIIB (S. Cerevisiae) (BRF1) interaction partners

  1. in Drosophila the alternative TRF1/BRF complex appears responsible for the initiation of all known classes of Pol III transcription

Mouse (Murine) BRF1 Homolog, Subunit of RNA Polymerase III Transcription Initiation Factor IIIB (S. Cerevisiae) (BRF1) interaction partners

  1. Brf1 (zfp36l1 (show ZFP36L1 Antibodies)), an AU-rich element mRNA-binding protein from the Zfp36 (show ZFP36 Antibodies) protein family, operates downstream of FGF/Erk MAP kinase (show MAPK1 Antibodies) signaling to regulate pluripotency and cell fate decision making in mouse embryonic stem cells.

  2. Alcohol induces RNA polymerase III-dependent transcription through c-Jun (show JUN Antibodies) by co-regulating TATA-binding protein (TBP (show TBP Antibodies)) and Brf1 (show ZFP36L1 Antibodies) expression.

  3. Phosphorylation at serine-92 and serine-203 is involved in BRF1 protein stability; high BRF1 protein levels are required to reinstate decay upon dissipation of the stabilizing signal.

Human BRF1 Homolog, Subunit of RNA Polymerase III Transcription Initiation Factor IIIB (S. Cerevisiae) (BRF1) interaction partners

  1. BRF1 mutations that reduce protein activity cause neurodevelopmental anomalies, suggesting that BRF1-mediated Pol III transcription is required for normal cerebellar and cognitive development.

  2. hnRNP F (show HNRNPF Antibodies) is a co-factor in a subset of tristetraprolin (show ZFP36 Antibodies)/BRF1 (show ZFP36L1 Antibodies)/BRF2 (show ZFP36L2 Antibodies)-mediated mRNA decay.

  3. these observations are in favor of a cell- and context-dependent regulation of Tis11b (show ZFP36L1 Antibodies) by hypoxia, which then contributes to modulation of angiogenesis.

  4. Alcohol induces RNA polymerase III-dependent transcription through c-Jun (show JUN Antibodies) by co-regulating TATA-binding protein (TBP (show TBP Antibodies)) and Brf1 (show ZFP36L1 Antibodies) expression.

  5. results identify a human Pol III isoform and isoform-specific functions in the regulation of cell growth and transformation

  6. CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells.

  7. BRF1 accelerated mRNA decay and antagonized the stabilizing effect of PI3-kinase, while mutation of the zinc fingers abolished both function and ARE-binding activity. This approach identifies BRF1 as an essential regulator of ARE-dependent mRNA decay.

  8. Data report that protein kinase B (PKB/Akt (show AKT1 Antibodies)) stabilizes ARE transcripts by phosphorylating butyrate response factor (BRF1 (show ZFP36L1 Antibodies)) at serine 92.

  9. These results suggest a direct role of an RNA polymerase III transcription factor in the targeting process.

  10. depletion of endogenous TTP (show ADAMTS13 Antibodies) and BRF-1 (show ZFP36L1 Antibodies) proteins, or overexpression of dominant-negative mutant TTP (show ADAMTS13 Antibodies) proteins, impairs the localization of reporter AU-rich element mRNAs

BRF1 Antigen Profile

Protein Summary

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified.

Gene names and symbols associated with BRF1

  • CG31256 gene product from transcript CG31256-RA (Brf) antibody
  • zgc:73380 (zgc:73380) antibody
  • general transcription factor IIB (GTF2B) antibody
  • transcription factor iib (PFE0415w) antibody
  • transcription factor IIB (Tb09.160.4220) antibody
  • transcription factor IIb (PVX_097980) antibody
  • transcription factor IIB (CpipJ_CPIJ011738) antibody
  • transcription factor IIB (CpipJ_CPIJ014799) antibody
  • transcription factor IIB (CpipJ_CPIJ018710) antibody
  • Transcription factor iib (PKH_102450) antibody
  • transcription initiation factor IIB (gtf2b) antibody
  • BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1) antibody
  • BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (Brf1) antibody
  • BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (BRF1) antibody
  • BRF1, RNA polymerase III transcription initiation factor 90 subunit (Brf1) antibody
  • 2510002F24Rik antibody
  • brf antibody
  • BRF-1 antibody
  • BRF1 antibody
  • CG4155 antibody
  • CG5419 antibody
  • CG31256 antibody
  • DDBDRAFT_0189159 antibody
  • DDBDRAFT_0216283 antibody
  • DDB_0189159 antibody
  • DDB_0216283 antibody
  • Dmel\\CG31256 antibody
  • GTF2B antibody
  • GTF3B antibody
  • hBRF antibody
  • mTFIIIB90 antibody
  • TAF3B2 antibody
  • TAF3C antibody
  • TAFIII90 antibody
  • TF3B90 antibody
  • TFIIIB antibody
  • TFIIIB90 antibody
  • ZFP36L1 antibody

Protein level used designations for BRF1

Brf-PA , Brf-PB , CG31256-PA , CG31256-PB , RNA Pol III transcription factor , UPF0468 protein C16orf80 homolog , transcription initiation factor IIB , general transcription factor IIB , transcription factor iib , transcription factor IIB , transcription factor IIb , Transcription factor iib , general transcription factor TFIIB , BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) , transcription initiation factor IIIB , zinc finger protein 36, C3H type-like 1 , B-related factor 1 , BRF-1 , hTFIIIB90 , transcription factor IIIB 90 kDa subunit , B - related factor 1 , BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB , TATA box binding protein (TBP)-associated factor 3C , TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2 , TBP - associated factor, RNA polymerase III, 90kD , general transcription factor IIIB, 90kD subunit

GENE ID SPECIES
42087 Drosophila melanogaster
393784 Danio rerio
694770 Macaca mulatta
743356 Pan troglodytes
812860 Plasmodium falciparum 3D7
3660552 Trypanosoma brucei brucei strain 927/4 GUTat10.1
5472512 Plasmodium vivax Sal-1
6044607 Culex quinquefasciatus
6048330 Culex quinquefasciatus
6052828 Culex quinquefasciatus
7321100 Plasmodium knowlesi strain H
8627902 Dictyostelium discoideum AX4
100173138 Pongo abelii
423503 Gallus gallus
453212 Pan troglodytes
490883 Canis lupus familiaris
72308 Mus musculus
2972 Homo sapiens
299347 Rattus norvegicus
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