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anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) Antibodies

BUB3 encodes a protein involved in spindle checkpoint function. Additionally we are shipping BUB3 Proteins (8) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
BUB3 9184 O43684
BUB3 361662  
BUB3 12237 Q9WVA3
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Top anti-BUB3 Antibodies at antibodies-online.com

Showing 10 out of 95 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Chicken Rabbit Un-conjugated IF, WB WB Suggested Anti-BUB3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: PANC1 cell lysate WB Suggested Anti-BUB3  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500  Positive Control: PANC1 cell lysate.BUB3 is strongly supported by BioGPS gene expression data to be expressed in PANC1 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Mouse Un-conjugated BI, WB Western blot analysis of Bub3 on a SW13 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Mouse Anti-Bub3 antibody. Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 well imaging plate at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the Mouse Anti-Bub3 antibody. The second step reagent was FITC goat anti mouse Ig. The image was taken on a BD Pathway™ 855 bioimagin system using a 20x objective. This antibody also stains HeLa (ATCC CCL-2) and U-2 OS (ATCC HTB-96) cells and can be used with either fix/perm protocol. 50 μg Log in to see 5 to 6 Days
$205.42
Details
Human Rabbit Un-conjugated ICC, IHC (fro), IHC (p), WB Anti-Bub3 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue 100 μg Log in to see 4 to 6 Days
$200.00
Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated IHC (p), WB Human Spleen (formalin-fixed, paraffin-embedded) stained with BUB3 antibody ABIN213913 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. 50 μg Log in to see 8 to 10 Days
$451.00
Details
Human Rabbit Un-conjugated IF, IHC (p), WB Immunohistochemistry (IHC) analyzes of Bub3 antibody in paraffin-embedded human brain tissue. Western blot (WB) analysis of Bub3 antibody in extracts from HeLa cells. 0.1 mg Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated ELISA, WB 100 μL Log in to see 8 to 10 Days
$478.50
Details
Human Rabbit Un-conjugated IC, IF, IP, IHC, WB Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 9 Days
$487.50
Details
Human Rabbit Un-conjugated IHC (p), WB 50 μg Log in to see 6 to 8 Days
$500.50
Details
Human Rabbit Un-conjugated WB 50 μg Log in to see 8 to 10 Days
$551.83
Details

BUB3 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-BUB3 Antibodies

  1. Human Monoclonal BUB3 Primary Antibody for BI, WB - ABIN968647 : Martinez-Exposito, Kaplan, Copeland, Sorger: Retention of the BUB3 checkpoint protein on lagging chromosomes. in Proceedings of the National Academy of Sciences of the United States of America 1999 (PubMed)
    Show all 2 references for ABIN968647

  2. Chicken Polyclonal BUB3 Primary Antibody for IF, WB - ABIN2787758 : Vaclavicek, Bermejo, Wappenschmidt, Meindl, Sutter, Schmutzler, Kiechle, Bugert, Burwinkel, Bartram, Hemminki, Försti: Genetic variation in the major mitotic checkpoint genes does not affect familial breast cancer risk. in Breast cancer research and treatment 2007 (PubMed)

More Antibodies against BUB3 Interaction Partners

Human Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) interaction partners

  1. We show that kinetochore recruitment of BUBR1 (show BUB1B Antibodies) and BUB3 by BUB1 (show BUB1 Antibodies) is dispensable for SAC (show ADCY10 Antibodies) activation

  2. a mutual antagonism exists between Bub3 and dynein to ensure optimal KT-MT attachments

  3. the mechanisms of BUB3 in mitotic checkpoint signaling

  4. Data show sequential multisite regulation of the microtubule-associated protein KNL1 (show CASC5 Antibodies)-kinase-adaptor complex BUB1 (show BUB1 Antibodies)/BUB3 interaction.

  5. Data indicate that cell cycle protein Bub3-mediated kinetochore recruitment of BubR1 (show BUB1B Antibodies) kinase enhances mitotic checkpoint signaling.

  6. Results suggest that these USP7 (show USP7 Antibodies)-dependent effects are mediated by decreased levels of spindle assembly checkpoint (SAC (show ADCY10 Antibodies)) component Bub3.

  7. Mutations targeting the Bub3 interface prevent kinetochore recruitment of the SAC (show ADCY10 Antibodies) kinase Bub1 (show BUB1 Antibodies). Crucially, they also cause a checkpoint defect, showing that recognition of phosphorylated targets by Bub3 is required for checkpoint signaling

  8. Results showed that neither variants in BUB3 nor variants in MAD2L1 (show MAD2L1 Antibodies) caused any significant effect on the risk of breast cancer

  9. Mutations in BUB1 (show BUB1 Antibodies) and BUB3 cause mosaic variegated aneuploidy and increase the risk of colorectal cancer at a young age.

  10. These results indicate that Bub3 and Cdc20 (show CDC20 Antibodies) play additional roles in the integration of cell cycle arrest as transcriptional repressors.

Mouse (Murine) Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) interaction partners

  1. as a member of the spindle assembly checkpoint, Bub3 is required for regulation of both meiosis I and II, and is potentially involved in kinetochore-microtubule attachment in mammalian oocytes

  2. Cenpa (show CENPA Antibodies), Cenpb (show CENPB Antibodies), and Bub3, but not Cenpc (show CENPC1 Antibodies), interacted with PARP-1 (show PARP1 Antibodies)

  3. Haploinsufficiency of Bub3 causes a slight increase in chromosome instability but is not clearly associated with a noticeable rise in the probability of tumor formation.

  4. subpopulation of dynein, containing the DYNLT3 (show DYNLT3 Antibodies) light chain, may be important for chromosome congression, in addition to having a role in the transport of checkpoint proteins from the kinetochore to the spindle pole

BUB3 Antigen Profile

Protein Summary

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene names and symbols associated with BUB3

  • BUB3 mitotic checkpoint protein (BUB3) antibody
  • BUB3 mitotic checkpoint protein (Bub3) antibody
  • BUB3 mitotic checkpoint protein (bub3) antibody
  • budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) (Bub3) antibody
  • budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3) antibody
  • Aa2-050 antibody
  • AU019800 antibody
  • AU021329 antibody
  • AU043350 antibody
  • AW146323 antibody
  • BUB3L antibody
  • C78067 antibody
  • hBUB3 antibody
  • xbub3 antibody

Protein level used designations for BUB3

BUB3 budding uninhibited by benzimidazoles 3 homolog , budding uninhibited by benomyl , budding uninhibited by benzimidazoles 3 homolog , mitotic checkpoint component , mitotic checkpoint protein BUB3 , WD repeat protein Bub3 , WD repeat type I transmembrane protein A72.5

GENE ID SPECIES
9184 Homo sapiens
361662 Rattus norvegicus
399106 Xenopus laevis
12237 Mus musculus
423949 Gallus gallus
539594 Bos taurus
477857 Canis lupus familiaris
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