anti-DEAD Box Protein 4 (DDX4) Antibodies

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. Additionally we are shipping DDX4 Kits (8) and DDX4 Proteins (6) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
DDX4 54514 Q9NQI0
DDX4 13206 Q61496
DDX4 310090 Q64060
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Top anti-DDX4 Antibodies at antibodies-online.com

Showing 10 out of 98 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of anti-DDX4 Antibody (C-term) (ABIN388152) in K562 cell line lysates (35 µg/lane). DDX4 (arrow) was detected using the purified polyclonal antibody (1:60 dilution). Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term) (ABIN388152), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Chicken Rabbit Un-conjugated IF (p), IHC (p), WB Formalin-fixed and paraffin embedded rat ovarian tissue labeled with Anti-DDX4/MVH Polyclonal Antibody, Unconjugated (ABIN701365) at 1:300 followed by conjugation to the secondary antibody and DAB staining Paraformaldehyde-fixed, paraffin embedded mouse testis, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes,  Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with DDX4 Polyclonal Antibody (bs-3597R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. 100 μL Log in to see 3 to 7 Days
$307.45
Details
Human HOST_Human Un-conjugated IHC (f), WB The R11-A3 Fab was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with immobilized recombinant human VASA/DDX4 protein. Western Blot analysis of VASA/DDX4 in mouse embryonic stem (ES) cell line. Lysate from ES-E14TG2a cells (ATCC CRL-1821) was probed with Purified Human anti-VASA/DDX4 Fab at titrations of 0.25 (lanes 1), 0.125 (lanes 2), and 0.062 µg/ml (lanes 3). VASA/DDX4 is identified as a band of 76 kDa in the mouse ES cells. Western Blot validation of VASA/DDX4 by RNAi in mouse ES cell line. Lysates from ES-E14TG2a cells (24 hour, lane 1, and 48 hour, lane 2) and VASA/DDX4 RNAi-transfected ES-E14TG2a cells (24 hour, lane 2, and 48 hour, lane 4) were probed with Purified Human anti-VASA/DDX4 Fab at 1.0 µg/ml. Down-regulation of VASA/DDX4 expression is evident in the RNAi-transfected cells. Purified Mouse anti-Actin monoclonal antibody (ABIN968902 or ABIN968903, 1:2000) was the gel-loading control. 0.1 mg Log in to see 6 to 8 Days
$523.65
Details
Chicken Rabbit FITC IF (p)   100 μL Log in to see 14 to 21 Days
$417.45
Details
Human Mouse Un-conjugated ICC, FACS, IHC, ELISA, WB Figure 1: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple) . Figure 2: Immunofluorescence analysis of MSCs (left) and NTERA-2 (right) cells using DDX4 mouse mAb (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. 100 μL Log in to see 2 to 3 Days
$393.80
Details
Human Rabbit Un-conjugated ICC, IHC (fro), IHC (p), WB Anti-DDX4/MVH antibody, ICC ICC: MCF-7 Cell Anti-DDX4/MVH antibody, IHC(P) IHC(P): Rat Ovary Tissue 100 μg Log in to see 4 to 6 Days
$200.00
Details
Human Rabbit Un-conjugated WB DDX4 Antibody (N-term)  western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow). DDX4 Antibody (N-term)  western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Mouse Un-conjugated EIA, IF, IHC (p), WB 0.1 mL Log in to see 6 to 8 Days
$390.50
Details
Dog Rabbit Un-conjugated IHC (p) Anti-DDX4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody  ABIN960130 concentration 5 ug/ml. 50 μg Log in to see 7 to 9 Days
$451.00
Details

DDX4 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-DDX4 Antibodies

  1. Human Monoclonal DDX4 Primary Antibody for IHC (f), WB - ABIN967664 : Abdelhaleem: RNA helicases: regulators of differentiation. in Clinical biochemistry 2005 (PubMed)
    Show all 6 Pubmed References

  2. Human Monoclonal DDX4 Primary Antibody for ICC, FACS - ABIN969084 : Castrillon, Quade, Wang, Quigley, Crum: The human VASA gene is specifically expressed in the germ cell lineage. in Proceedings of the National Academy of Sciences of the United States of America 2000 (PubMed)
    Show all 4 Pubmed References

  3. Human Monoclonal DDX4 Primary Antibody for FACS, IF - ABIN965984 : Jones: Predicability (ease of predication) as semantic substrate of imageability in reading and retrieval. in Brain and language 2002 (PubMed)
    Show all 4 Pubmed References

  4. Chicken Polyclonal DDX4 Primary Antibody for IF (p) - ABIN701372 : Imus, Roe, Charter, Durrant, Jensen: Transfer and detection of freshly isolated or cultured chicken (Gallus gallus) and exotic species' embryonic gonadal germ stem cells in host embryos. in Zoological science 2014 (PubMed)

More Antibodies against DDX4 Interaction Partners

Human DEAD Box Protein 4 (DDX4) interaction partners

  1. Results suggest that DDX4 positively regulates cell cycle progression of diverse somatic-derived blood cancer cells, implying its broad contributions to the cancer cell phenotype.

  2. DDX4 cell-free seminal mRNA was absent in 44% of Sertoli cell-only syndrome cases diagnosed by testicular histopathology, but present in all patients presenting with maturation arrest or hypospermatogenesis

  3. The 20-mer (show MERTK Antibodies) peptide (residues 228-247) of human DDX-4, an ATP-dependent RNA helicase (show DDX52 Antibodies) known to regulate germ cell development, binds to a unique shallow binding surface on RanBPM (show RANBP9 Antibodies) formed by highly conserved loops on the surface of the beta-sheet with two aspartates on one end, a positive patch on the opposite end, and a tryptophan lining at the bottom of the surface.

  4. Data show that intrinsically disordered N terminus of DEAD-box RNA helicase (show DDX5 Antibodies) Ddx4 forms organelles in HeLa cells and in vitro.

  5. this study reports the first analysis and comparison of the expression patterns and colocalization of DDX4 and CD133 in 59 ovarian cancer patients via tissue microarray and immunofluorescence analysis to identify the expression patterns and relationship of those two markers in ovarian cancer cells.

  6. Data suggest that VASA shows differential expression in ovary; in resting primordial follicle reserve in childhood/pre-puberty, VASA is associated with Balbiani's body space in germ cell; once follicle enters growing pool, VASA is undetectable.

  7. Vasa/Ddx4 gene can be involved in regulation of ground and primed states of pluripotency

  8. overexpression of VASA and or DAZL (show DAZL Antibodies), in both embryonic and induced pluripotent stem cells promoted differentiation into primordial germ cells, and maturation and progression through meiosis was enhanced.

  9. Report the generation/characterization of embryonic stem cell lines carrying a VASA-pEGFP-1 reporter construct that expresses GFP in a population of differentiating cells showing expression of characteristic markers of primordial germ cells.

  10. The expression of VASA mRNA and protein was significantly decreased in the sperm of oligozoospermic men.

Mouse (Murine) DEAD Box Protein 4 (DDX4) interaction partners

  1. BHR also decreased the mRNA level of MVH in the bone marrow, and increased mRNA and protein level of MVH in the ovary o fpremature ovarian failure mice

  2. Hgher expression levels of ~55-60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles.

  3. In mouse oocytes the mitochondrion-originated germinal body-like structures accumulate MVH protein.

  4. The results indicate the presence of a developmentally regulated mechanism in which Hat1 (show HAT1 Antibodies) and p46 (show POLDIP3 Antibodies) control the physiology of the chromatoid body by acetylation of MVH.

  5. Vasa/Ddx4 gene can be involved in regulation of ground and primed states of pluripotency

  6. SPSB1 (show SPSB1 Antibodies) and SPSB4 bind strongly to both Par-4 (show F2RL3 Antibodies) and VASA peptides.

  7. MVH plays crucial roles in the early phase of the ping-pong amplification cycle

  8. The RNA helicase Vasa is another essential protein in germline development and arginine methylation is conserved across phyla

  9. Data show that RanBPM (show RANBP9 Antibodies) and mouse vasa homolog (Mvh) are closely associated with perinuclear RNA-protein complexes and chromatoid bodies, and suggests a functional relationship between translational regulation and microtubule nucleation during meiosis.

  10. Dazl (show DAZL Antibodies) stimulates translation via the Mvh 3'-UTR (show UTS2R Antibodies). Dazl (show DAZL Antibodies)-mediated regulation of Mvh translation is crucial for mammalian spermatogenesis

Pig (Porcine) DEAD Box Protein 4 (DDX4) interaction partners

  1. These results demonstrate that testicular cell-surface DDX4-immunoreactive cells are not germ cells and constitute a distinct subpopulation that is different from gonocytes.

  2. Porcine Vasa Homolog (pvh) is specifically expressed in germ cell lineages, and can be a useful marker for germ cell development in pigs.

Cow (Bovine) DEAD Box Protein 4 (DDX4) interaction partners

  1. Bone morphogenetic protein 4 (show BMP4 Antibodies) and retinoic acid trigger bovine VASA homolog expression in differentiating bovine induced pluripotent stem cells.

  2. VASA was highly represented in the testis and the ovary, and still present in the oocyte from antral follicles.

  3. The expression of VASA in bovine fetuses is reported.

Xenopus laevis DEAD Box Protein 4 (DDX4) interaction partners

  1. The RNA helicase (show DDX46 Antibodies) Vasa is another essential protein in germline development and arginine methylation is conserved across phyla

  2. XVLG1 protein in pPGC is not sufficient to increase the number of primordial germ cells in the tadpoles

  3. Xdsg protein in pPGCs may play an important role in the formation of PGCs by regulating the production of XVLG1 protein

DDX4 Antigen Profile

Protein Summary

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with DDX4

  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4) antibody
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4) antibody
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (ddx4) antibody
  • AV206478 antibody
  • Mvh antibody
  • RVLG antibody
  • vasa antibody
  • vlg1 antibody

Protein level used designations for DDX4

DEAD box protein 4 , DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 , probable ATP-dependent RNA helicase DDX4 , vasa homolog , D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 4 , DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 4 , DEAD box polypeptide 4 , mvh / m'vasa , Cvh , vasa-like protein , putative ATP-dependent RNA helicase DDX4 , vasa-like gene protein , xVLG1

GENE ID SPECIES
54514 Homo sapiens
487211 Canis lupus familiaris
13206 Mus musculus
395447 Gallus gallus
431672 Sus scrofa
493725 Bos taurus
310090 Rattus norvegicus
398019 Xenopus laevis
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