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anti-Extra Spindle Poles Like 1 (ESPL1) Antibodies

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. Additionally we are shipping ESPL1 Kits (7) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ESPL1 9700 Q14674
ESPL1 315330  
ESPL1 105988 P60330
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Top anti-ESPL1 Antibodies at antibodies-online.com

Showing 10 out of 185 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated EIA, IHC (p) 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated DB, IHC (p) Dot blot analysis of anti-hSeparase-S801 Phospho-specific Pab (ABIN389654) on nitrocellulose membrane.  50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5 µg/mL. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated IF, IHC, ELISA, WB Western blot analysis of extracts from 293 cells, treated with EGF 200ng/ml 30', using SEPARASE (Ab-801) Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Ab-801) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated IF, IHC, ELISA, WB Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Mouse Un-conjugated IHC (p), ELISA, WB ESPL1 monoclonal antibody (M01), clone 6H6 Western Blot analysis of ESPL1 expression in Hela S3 NE . Immunoperoxidase of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] 100 μg Log in to see 9 Days
$430.00
Details
Human Rabbit Un-conjugated IF, IHC (p), ELISA, WB 50 μL Log in to see 8 to 10 Days
$451.00
Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of Separase (pS801) staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 9 Days
$575.00
Details
Human Rabbit Un-conjugated IF, IHC (p), WB Immunohistochemistry analyzes of Separase antibody in paraffin-embedded human colon carcinoma tissue. Western blot analysis of Separase antibody (9AP20283PU-N) in extracts from 293 cells. 0.1 mg Log in to see 6 to 8 Days
$390.50
Details
Human Mouse Un-conjugated DB 50 μg Log in to see 7 to 9 Days
$285.71
Details
Human Rabbit Un-conjugated WB 100 μL Log in to see 14 Days
$448.47
Details

ESPL1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-ESPL1 Antibodies

  1. Human Monoclonal ESPL1 Primary Antibody for IHC (p), ELISA - ABIN564183 : Zhang, Ge, Meyer, Sethi, Basu, Pradhan, Zhao, Li, Cai, El-Naggar, Baladandayuthapani, Kittrell, Rao, Medina, Pati: Overexpression of Separase induces aneuploidy and mammary tumorigenesis. in Proceedings of the National Academy of Sciences of the United States of America 2008 (PubMed)
    Show all 7 references for ABIN564183

  2. Human Polyclonal ESPL1 Primary Antibody for EIA, IHC (p) - ABIN358223 : Chestukhin, Pfeffer, Milligan, DeCaprio, Pellman: Processing, localization, and requirement of human separase for normal anaphase progression. in Proceedings of the National Academy of Sciences of the United States of America 2003 (PubMed)
    Show all 5 references for ABIN358223

  3. Human Polyclonal ESPL1 Primary Antibody for IF, IHC - ABIN1532260 : Zou, McGarry, Bernal, Kirschner: Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis. in Science (New York, N.Y.) 1999 (PubMed)

  4. Human Polyclonal ESPL1 Primary Antibody for DB, IHC (p) - ABIN389654 : Haaß, Stehle, Nittka, Giehl, Schrotz-King, Fabarius, Hofmann, Seifarth: The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib. in PLoS ONE 2012 (PubMed)

More Antibodies against ESPL1 Interaction Partners

Human Extra Spindle Poles Like 1 (ESPL1) interaction partners

  1. The assay was used to quantify Separase proteolytic activity in leukemic cell lines and peripheral blood samples from leukemia patients.

  2. Recruitment and activation of separase at centrosomes are two distinct steps that do not require microtubules.

  3. Separase protein levels decrease and Separase proteolytic activity increases exclusively in b3a2 p210BCR-ABL (show ABL1 Antibodies)-positive cell lines under Imatinib treatment.

  4. Separase is an oncogene (show RAB1A Antibodies) whose overexpression induces tumorigenesis, and indicate that Separase overexpression and aberrant nuclear localization are common in many tumor types and may predict outcome in some human malignancies.

  5. High ESPL1 mRNA expression was associated with luminal B breast cancers.

  6. Data indicate that separase is subject to native-state cis (show CISH Antibodies)/trans isomerization by peptidyl-prolyl-isomerase Pin1 (show PIN1 Antibodies).

  7. Mutation of the homologous position in PTTG1 (show PTTG1 Antibodies) (H(134)) switched PTTG1 (show PTTG1 Antibodies) from an inhibitor into an activator of ESP1 (show AES Antibodies).

  8. By consecutively acting as a protease and a cdk1 (show CDK1 Antibodies) inhibitor, separase coordinates two key processes to achieve simultaneous and abrupt separation of sister chromatids.

  9. Kendrin (show PCNT Antibodies) is a novel and crucial substrate for separase (ESPL1) at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis.

  10. SMC3 (show SMC3 Antibodies) and separase are upregulated and securin (show PTTG1 Antibodies) is downregulated in malignant transformation of BEAS-2B cells induced by coal tar (show RBM8A Antibodies) pitch smoke extracts.

Mouse (Murine) Extra Spindle Poles Like 1 (ESPL1) interaction partners

  1. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway.

  2. Separase-overexpressing mammary cells are not only susceptible to chromosomal missegregation-induced aneuploidy but also other genetic instabilities

  3. With the continual loss of cohesins from chromosomes that occurs throughout the natural reproductive lifespan, tight regulation of separase in oocytes may be particularly important to maintain cohesion and prevent aneuploidy.

  4. Separase act synergistically with loss of p53 (show TP53 Antibodies) in the initiation and progression of B- and T- cell lymphomas

  5. Separase phospho-regulation is critical for genome stability in oogenesis.

  6. Separase phosphorylation act redundantly to prevent sister chromatid separation.

  7. In embryonic fibroblasts, Separase depletion blocks sister chromatid separation but does not prevent other aspects of mitosis, cytokinesis, or chromosome replication.

  8. Proteolytic activity of separase is therefore essential for Rec8 (show REC8 Antibodies)'s removal from chromosome arms and for chiasma resolution but not for PBE (show EHHADH Antibodies).

  9. inhibitory phosphorylation of separase plays a critical role in the maintenance of sister chromatid cohesion and genome stability in proliferating postmigratory primordial germ cells

  10. These results collectively suggest that Separase is an oncogene (show RAB1A Antibodies), whose overexpression alone in mammary epithelial cells is sufficient to induce aneuploidy and tumorigenesis in a p53 (show TP53 Antibodies) mutant background.

ESPL1 Antigen Profile

Protein Summary

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21\; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009

Gene names and symbols associated with ESPL1

  • extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1) antibody
  • extra spindle poles like 1 (espl1) antibody
  • extra spindle pole bodies homolog 1 (S. cerevisiae) (Espl1) antibody
  • extra spindle poles-like 1 (S. cerevisiae) (Espl1) antibody
  • AL024103 antibody
  • AU045071 antibody
  • Cerp antibody
  • ESP1 antibody
  • mp:zf637-3-001691 antibody
  • PRCE antibody
  • SEPA antibody
  • SSE antibody

Protein level used designations for ESPL1

extra spindle poles like 1 , cds , separase , cease&desist , zf637-3-001691 , caspase-like protein ESPL1 , extra spindle poles-like 1 protein , separin , separin, cysteine protease , Cut1/ESP1 related protein

GENE ID SPECIES
100063779 Equus caballus
337864 Danio rerio
9700 Homo sapiens
425536 Gallus gallus
607879 Canis lupus familiaris
506740 Bos taurus
315330 Rattus norvegicus
105988 Mus musculus
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