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anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) Antibodies

GAD2 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. Additionally we are shipping GAD65 Kits (24) and GAD65 Proteins (17) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
GAD2 24380 Q05683
GAD2 2572 Q05329
GAD2 14417 P48320
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Top anti-GAD65 Antibodies at antibodies-online.com

Showing 10 out of 222 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated EIA, IF, IHC (p), WB Confocal immunofluorescent analysis of GAD2 / GAD65 Antibody (Center) Cat.-No AP51755PU-N with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with GAD2 / GAD65 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining. 0.4 mL Log in to see 6 to 8 Days
$401.50
Details
Mouse Mouse Un-conjugated FACS, IP, RIA, WB 0.1 mL Log in to see 5 to 6 Days
$455.84
Details
Dog Goat Un-conjugated WB 0.1 mg Log in to see 6 to 8 Days
$379.50
Details
Human Mouse Un-conjugated IHC (fro), IP, WB Western blot analysis of GAD65. Lysates from rat brain cortex were probed with purified anti-GAD65 (clone GAD-6) at a concentration of 5.0 (lane 1), 2.0 (lane 2) or 0.5 µg/ml (lane 3). GAD65 is identified as a protein of ~65 kDa. 0.1 mg Log in to see 5 to 6 Days
$329.87
Details
Rat Mouse Un-conjugated ICC, IP, IHC, ELISA, WB dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2) Immunolabeling of cultured hippocampus neurons (dilution 1 : 500). 100 μg Log in to see 5 to 6 Days
$444.68
Details
Human Goat Un-conjugated ELISA, WB ABIN185388 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 100 μg Log in to see 4 to 5 Days
$318.20
Details
Human Rabbit Un-conjugated IF, IHC (p), WB GAD2 Antibody (Center)  western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow). GAD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry.  Clinical relevance has not been evaluated. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated IHC (p), WB WB Image Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE antibody diluted at 1:10000 IHC-P Image GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody [C2C3], C-term , dilution: 1:500. 100 μL Log in to see 2 to 3 Days
$358.60
Details
Human Rabbit Un-conjugated IHC (p), WB Anti-GAD65 antibody, IHC(P) IHC(P): Rat Brain Tissue Anti-GAD65 antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate 100 μg Log in to see 4 to 6 Days
$200.00
Details
Human Mouse Un-conjugated WB GAD2 Antibody  western blot analysis in rat brain tissue lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow). 0.1 mL Log in to see 10 to 11 Days
$324.50
Details

GAD2 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-GAD65 Antibodies

  1. Human Monoclonal GAD65 Primary Antibody for IHC (fro), IP - ABIN967657 : Bu, Erlander, Hitz, Tillakaratne, Kaufman, Wagner-McPherson, Evans, Tobin: Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. in Proceedings of the National Academy of Sciences of the United States of America 1992 (PubMed)
    Show all 7 references for ABIN967657

  2. Mouse (Murine) Monoclonal GAD65 Primary Antibody for FACS, IP - ABIN1042632 : Steed, Gilliam, Harris, Lernmark, Hampe: Antigen presentation of detergent-free glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier protein. in Journal of immunological methods 2008 (PubMed)
    Show all 3 references for ABIN1042632

  3. Human Polyclonal GAD65 Primary Antibody for EIA, IF - ABIN952422 : Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. in Diabetes Care 2010 (PubMed)
    Show all 2 references for ABIN952422

  4. Human Polyclonal GAD65 Primary Antibody for ELISA, WB - ABIN185388 : Meyre, Boutin, Tounian, Deweirder, Aout, Jouret, Heude, Weill, Tauber, Tounian, Froguel: Is glutamate decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? in The Journal of clinical endocrinology and metabolism 2005 (PubMed)

  5. Rat (Rattus) Monoclonal GAD65 Primary Antibody for ICC, IP - ABIN1742417 : Coley, Ruffin, Moss, Hopfer, Boron: Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons. in Neuroscience 2013 (PubMed)

More Antibodies against GAD65 Interaction Partners

Rhesus Monkey Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. Results describe the gross distribution patterns of GAD65 in human and rhesus monkey thalami and show that they are nearly identical.

Zebrafish Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. GAD65 mRNA was found in the periaqueductal gray.

Human Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. UV exposed hydrogen peroxide produces hydroxyl radical that may cause protein damage (GAD-65) to the extent of generating neo-epitopes on the molecule, making it immunogenic.

  2. we investigated the relationship between serum glutamic acid decarboxylase (GAD) autoantibody (Ab) levels and single nucleotide polymorphisms (SNPs) of the glutamic acid de-carboxylase 2 (GAD2) 5'-untranslated region

  3. GAD (show GAD1 Antibodies) autoantibodies were associated with risk of developing type 1 diabetes.

  4. Kaplan-Meier survival curves estimated a worst pancreas graft survival for patients with positive IA-2 (show PTPRN Antibodies) antibodies versus those patients with negative auto-antibodies and GAD65+ auto-antibodies after simultaneous pancreas kidney transplantation.

  5. Plasma GAD65 qualifies as a marker for early beta-cell loss after intraportal transplantation.

  6. Cohort study shows that mean GAD65 mRNA levels in the prefrontal cortex area 9 are not altered in subjects with schizophrenia but are lower in subjects with schizoaffective disorder

  7. Data suggest that women with gestational diabetes who develop autoantibodies against GAD2 (glutamate decarboxylase 2) exhibit higher blood glucose levels, insulin (show INS Antibodies) resistance, and impaired insulin (show INS Antibodies) secretion from beta cells in the postpartum period.

  8. Phosphate-activated glutaminase (show GLS2 Antibodies) and GAD65/67 concentrations are compared in Alzheimer's disease cerebellum versus normal cerebellum controls

  9. GAD2 SNPs significantly associate with early-onset obsessive compulsive disorder.

  10. The decreased amount of GAD65 and GAD67 (show GAD1 Antibodies) suggests the decreased synthesis of neurotransmitter and basic GABA pools that indicates insufficient functioning of the GABA system in the cerebellar cortex of Alzheimer's disease patients.

Mouse (Murine) Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. Increased activity due to bicuculline preferentially up-regulated GAD65. This was reduced by AP5, a selective blocker of Ca2 (show CA2 Antibodies)+-permeable ion channels and NMDA-Rs, and by U0126, KN93, and K252a, affecting BDNF (show BDNF Antibodies) pathways.

  2. Data show that miRNAs are involved in regulating the expression of the major type 1 diabetes (T1D) autoantigens IA-2 (show PTPRN Antibodies), IA-2beta (show PTPRN2 Antibodies), and GAD65 enzyme.

  3. Results indicate the significant contribution of not only GAD65, GAD67 (show GAD1 Antibodies) and VGAT (show SLC32A1 Antibodies)-mediated GABAergic but also glycinergic transmissions to both palate and abdominal wall formations

  4. Analysis of GAD65/67 single and double knock-out embryos revealed that the two GADs play complementary (inhibitory) roles in GnRH migration ultimately modulating the speed and/or direction of GnRH migration.

  5. Results show that GAD65 is required for efficient GABAergic synaptic transmission and plasticity, and for maintaining extracellular GABA at (show ABAT Antibodies) a level needed for associative plasticity at cortical inputs in the lateral amygdala

  6. Menin (show MEN1 Antibodies) regulates spinal glutamate (show GRIN1 Antibodies)-GABA balance through GAD65 contributing to neuropathic pain.

  7. Our data suggest a role of GAD65-mediated GABA synthesis in the encoding of circadian information to fear memory

  8. Autoimmunity to GAD65 may play a role in the development of Stiff person syndrome.

  9. Prenatal and postnatal maternal effects influence GAD65 and GAD67 (show GAD1 Antibodies) gene expression depending on the age of the offspring.

  10. These findings indicate novel, differential roles for GluN2A (show GRIN2A Antibodies), B and D receptors and for GAD65-mediated GABA in the regulation of individual topographies of orofacial movement

GAD65 (GAD2) Antigen Profile

Protein Summary

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Gene names and symbols associated with GAD2

  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2) antibody
  • glutamate decarboxylase 2 (GAD2) antibody
  • glutamate decarboxylase 2 (PTRG_06148) antibody
  • glutamate decarboxylase 2 (ARALYDRAFT_894224) antibody
  • glutamate decarboxylase 2 (Gad2) antibody
  • glutamate decarboxylase 2 (LOC100344727) antibody
  • glutamate decarboxylase 2 (gad2) antibody
  • glutamic acid decarboxylase 2 (Gad2) antibody
  • 6330404F12Rik antibody
  • F12P19.12 antibody
  • F12P19_12 antibody
  • GAD(65) antibody
  • Gad-2 antibody
  • gad65 antibody
  • glutamate decarboxylase 2 antibody
  • zgc:112198 antibody

Protein level used designations for GAD2

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 , glutamic acid decarboxylase , 65 kDa glutamic acid decarboxylase , GAD-65 , Glutamate decarboxylase 2 (islet) , glutamate decarboxylase 65 kDa isoform , glutamic acid decarboxylase 2 , glutamic acid decarboxylase 65 , Glutamate decarboxylase-2 (pancreas)

GENE ID SPECIES
466026 Pan troglodytes
512459 Bos taurus
613029 Macaca mulatta
4836947 Scheffersomyces stipitis CBS 6054
6344405 Pyrenophora tritici-repentis Pt-1C-BFP
9324534 Arabidopsis lyrata subsp. lyrata
24380 Rattus norvegicus
100344727 Oryctolagus cuniculus
842908 Arabidopsis thaliana
550403 Danio rerio
2572 Homo sapiens
487107 Canis lupus familiaris
395395 Gallus gallus
396929 Sus scrofa
14417 Mus musculus
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