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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) Antibodies

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. Additionally we are shipping Glutamate-Cysteine Ligase, Catalytic Subunit Kits (24) and Glutamate-Cysteine Ligase, Catalytic Subunit Proteins (12) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
GCLC 2729 P48506
GCLC 14629 P97494
GCLC 25283 P19468
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Top anti-Glutamate-Cysteine Ligase, Catalytic Subunit Antibodies at antibodies-online.com

Showing 10 out of 134 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IHC, WB WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta Immunohistochemistry with Spleen tissue at an antibody concentration of 5µg/ml using anti-GCLC antibody (ARP54577_P050) 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/mlPositive Control: Human Liver 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated EIA, FACS, IF, IHC (p), WB Flow cytometric analysis of Jurkat cells using GCLC / GLCLC Antibody (N-term) Cat.-No AP51800PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. Confocal immunofluorescent analysis of GCLC / GLCLC Antibody (N-term) Cat.-No AP51800PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated FACS, IF, IHC (p), WB Confocal immunofluorescent analysis of GCLC Antibody (N-term) (ABIN655884) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Mouse Un-conjugated IF, ELISA, WB Immunofluorescence of monoclonal antibody to GCLC on HeLa cell. [antibody concentration 10 ug/ml] GCLC monoclonal antibody (M01), clone 3H1 Western Blot analysis of GCLC expression in A-431 . 100 μg Log in to see 9 Days
$430.00
Details
Cow Rabbit Un-conjugated EIA, IHC (p), IP, WB 50 μL Log in to see 6 to 8 Days
$500.50
Details
Human Rabbit Un-conjugated IHC (p), WB Human Brain, Cerebellum 50 μg Log in to see 8 to 10 Days
$676.50
Details
Human Rabbit Un-conjugated IHC (p), WB Anti-GCLC antibody, IHC(P) IHC(P): Human Rectal Cancer Tissue Anti-GCLC antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: Rat Heart Tissue Lysate Lane 4: Rat Lung Tissue Lysate 100 μg Log in to see 4 to 6 Days
$200.00
Details
Human Rabbit Un-conjugated IHC (p), WB Anti- GCLC antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue Anti- GCLC antibody, Western blotting All lanes: Anti GCLC  at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug Lane 3: HELA Cell Lysate at 40ug Lane 4: PC-12 Whole Cell Lysate at 40ug Lane 5: NRK Whole Cell Lysate at 40ug Lane 6: HEPA Whole Cell Lysate at 40ug Lane 7: A549 Whole Cell Lysate at 40ug Predicted bind size: 72KD Observed bind size: 72KD 100 μg Log in to see 4 to 6 Days
$240.00
Details
Human Rabbit Un-conjugated WB GCLC Antibody (Center)  western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow). GCLC Antibody (Center)  western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details

GCLC Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-Glutamate-Cysteine Ligase, Catalytic Subunit Antibodies

  1. Human Monoclonal GCLC Primary Antibody for IF, ELISA - ABIN561050 : Hardwick, Fisher, Canet, Lake, Cherrington: Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease. in Drug metabolism and disposition: the biological fate of chemicals 2010 (PubMed)
    Show all 6 references for ABIN561050

  2. Human Polyclonal GCLC Primary Antibody for EIA, FACS - ABIN952477 : Man, Baum, Fu, Chan, Lam, Hui, Leung, Wong: Genetic polymorphisms of Chinese patients with ischemic stroke and concurrent stenoses of extracranial and intracranial vessels. in Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2010 (PubMed)
    Show all 3 references for ABIN952477

  3. Human Polyclonal GCLC Primary Antibody for IHC, WB - ABIN2785780 : Bardag-Gorce, Oliva, Lin, Li, French, French: Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. in Experimental and molecular pathology 2011 (PubMed)
    Show all 2 references for ABIN2785780

  4. Cow (Bovine) Polyclonal GCLC Primary Antibody for WB - ABIN2774078 : Jönsson, Jönsson, Axmon, Littorin, Broberg: Influence of glutathione-related genes on symptoms and immunologic markers among vulcanization workers in the southern Sweden rubber industries. in International archives of occupational and environmental health 2008 (PubMed)

More Antibodies against Glutamate-Cysteine Ligase, Catalytic Subunit Interaction Partners

Fruit Fly (Drosophila melanogaster) Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) interaction partners

  1. Microarray analysis revealed that glutamate (show GRIN2A Antibodies) cysteine ligasec overexpression and thus enhanced glutathione production has a broad impact on gene expression that largely affects different processes in young and old flies.

  2. investigation of mutations in GCL modifier subunit and the GCL catalytic subunit that modify catalytic activity and lower glutathione levels

  3. Neuronal overexpression of GCLc in a long-lived background extended mean and maximum life spans up to 50%, without affecting the rate of oxygen consumption by the flies

  4. The reversibility of the dephosphorylation-dependent activation was indicated by the time-dependent inactivation of the in vitro activated Drosophila GCL, by preincubation with MgATP.

  5. that the longevity effects of GCLc are dependent on dosage and that there are specific tissues (mushroom bodies, motor neurons, and transverse muscle cells) particularly sensitive to the benefits of GCLc overexpression.

  6. GSH biosynthesis in the nucleus is associated with migration of only the GCLc subunit from the cytoplasm into the nucleus, and this migration requires the presence of an intact nuclear localization signal

Human Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) interaction partners

  1. miR-433 targets both catalytic (GCLc) and regulatory (GCLm) subunits of GCL.

  2. Data suggest expression of hepatocyte GCLC and GCLM (show GCLM Antibodies) can be regulated by dietary component; alpha-lipoic acid, a vitamin B complex nutrient, protects against oxidative stress/cytotoxicity induced by cadmium via restoration of GCLC and GCLM (show GCLM Antibodies) expression.

  3. Cigarette smoke-induced hypermethylation of the GCLC promoter is related to the initiation and progression of COPD (show ARCN1 Antibodies).

  4. GCLC and GSS (show GSS Antibodies) were expressed at higher levels in colon cancer tissue, as compared with normal mucosa.

  5. The Kaplan-Meier analysis shows that rs3736729 on GCLC presents a significant association with disease-free survival and overall survival.

  6. H2S upregulates GCLC and GSH and inhibits IL-1beta (show IL1B Antibodies) levels, which may be what mediates the beneficial effects of H2S-rich compounds in mitigating the pathogenesis of metabolic syndrome and atherosclerosis

  7. the CYP1A1 (show CYP1A1 Antibodies) (rs2606345, rs4646903, rs1048943), GCLC, AGT (show AGXT Antibodies), and AGTR1 (show AGTR1 Antibodies) genes was associated with pleuritis, empyema, acute respiratory distress syndrome, all PC and acute respiratory failure (ARF (show CDKN2A Antibodies)).

  8. A functional trinucleotide repeat polymorphism in the 5'-untranslated region of the glutathione biosynthetic gene GCLC is associated with increased risk for lung and aerodigestive tract cancers.

  9. These data strongly indicate a discrepancy between the regulation of GCLC and of GGT following the oxidative stress situation due to mitochondrial uncoupling.

  10. 1,25 (OH) vitamin D significantly upregulated expression of GCLC and GR and lowered secretion of IL-8 (show IL8 Antibodies) and MCP-1 (show CCL2 Antibodies) in high-glucose exposed U937 monocytes.

Mouse (Murine) Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) interaction partners

  1. A floxed Gclc mouse was generated and crossed with a transgenic mouse expressing Cre in the lens to generate the Lens Glutathione Synthesis Knockout mouse in which de novo GSH synthesis was completely abolished in the lens.

  2. Clinically relevant levels of TGF-beta1 (show TGFB1 Antibodies) suppresses GCLC and GCLM (show GCLM Antibodies) expression in mouse lung.

  3. Data show for the first time that GCLC may serve a dual role, as a surrogate marker for cellular redox state as well as malignant potential of melanoma cells.

  4. The impacts of four clinical missense mutations on GCLC enzymatic function in vivo and in vitro, was evaluated.

  5. tBHQ has beneficial effects on reducing hyperglycemia-induced kidney injury, which is associated with the enhanced expression of Nrf2 (show NFE2L2 Antibodies), and its downstream antioxidant HO-1 (show HMOX1 Antibodies) and gamma-GCS (show UGCG Antibodies) in the glomeruli of diabetic mice

  6. In first days of life luminescence measured was in all mice with distinct strain differences indicating NF-kappaB (show NFKB1 Antibodies), superoxide dismutase (show SOD1 Antibodies), gamma-glutamylcysteine synthetase, and antioxidant responsive element activity.

  7. Hypoxia decreased 2 key enzyme activities that regulate GSH synthesis, glutamate cysteine ligase (GCL) (E.C. 6.3.2.2) and glutathione synthase (show GSS Antibodies) (GS) (E.C. 6.3.2.3)

  8. activation of GSK-3beta (show GSK3b Antibodies) is a key mediator of the initial phase of acetaminophen-induced liver injury through modulating GCL and Mcl-1 (show MCL1 Antibodies) degradation, as well as JNK (show MAPK8 Antibodies) activation in liver

  9. These findings demonstrate that TAT (show TAT Antibodies)-mediated transduction of wild-type or dominant-inhibitory mutants of the Glutamate cysteine ligase (GCL) subunits is a viable means of manipulating cellular GCL activity.

  10. The GCLc protein was widely expressed throughout the embryo, and, in general, it co-localized with the Gclc mRNA.

Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) Antigen Profile

Protein Summary

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Gene names and symbols associated with GCLC

  • glutamate-cysteine ligase, catalytic subunit (GCLC) antibody
  • glutamate-cysteine ligase, catalytic subunit (gclc) antibody
  • Glutamate-cysteine ligase catalytic subunit (Gclc) antibody
  • gamma glutamylcysteine synthetase (LbGCS) antibody
  • gamma glutamylcysteine synthetase (gcsA) antibody
  • glutamate-cysteine ligase, catalytic subunit (LOC100049719) antibody
  • gamma-glutamylcysteine synthetase (GSH1) antibody
  • glutamate-cysteine ligase, catalytic subunit (Gclc) antibody
  • 2259 antibody
  • cb1049 antibody
  • CG2259 antibody
  • D9Wsu168e antibody
  • DDBDRAFT_0186120 antibody
  • DDBDRAFT_0231403 antibody
  • DDB_0186120 antibody
  • DDB_0231403 antibody
  • Dmel\\CG2259 antibody
  • DmGCLC antibody
  • DmGCS antibody
  • DmGCSh antibody
  • fe36e11 antibody
  • GCL antibody
  • GCLc antibody
  • GCS antibody
  • Gcsh antibody
  • Ggcs-hs antibody
  • GLCL antibody
  • GLCL-H antibody
  • Glclc antibody
  • MGC146287 antibody
  • wu:fe36e11 antibody

Protein level used designations for GCLC

glutamate--cysteine ligase catalytic subunit , glutamate-cysteine ligase, catalytic subunit , CG2259-PA , CG2259-PB , Gclc-PA , Gclc-PB , gamma-Glutamylcysteine synthetase , gamma-Glutamylcysteine synthetase catalytic subunit , gamma-glutamylcysteine ligase , glutamate cysteine ligase , glutamate-cysteine ligase , gamma glutamylcysteine synthetase , gamma-ECS , glutamate--cysteine ligase, chloroplastic , GCS heavy chain , gamma-glutamylcysteine synthetase , gamma GCS-HS , gamma-glutamylcysteine synthetase heavy subunit , Glutamylcysteine gamma synthetase light chain , glutamate-cysteine ligase catalytic subunit

GENE ID SPECIES
710508 Macaca mulatta
742732 Pan troglodytes
780264 Xenopus (Silurana) tropicalis
53581 Drosophila melanogaster
6073019 Laccaria bicolor S238N-H82
8624704 Dictyostelium discoideum AX4
100049719 Xenopus laevis
100137615 Xenopus laevis
543536 Solanum lycopersicum
2729 Homo sapiens
14629 Mus musculus
25283 Rattus norvegicus
326857 Danio rerio
609822 Canis lupus familiaris
512468 Bos taurus
421894 Gallus gallus
100726181 Cavia porcellus
100522018 Sus scrofa
100342383 Oryctolagus cuniculus
101114822 Ovis aries
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