anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibodies

The product of GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Additionally we are shipping GAPDH Proteins (74) and GAPDH Kits (45) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
Anti-Human GAPDH GAPDH 2597 P04406
Anti-Mouse GAPDH GAPDH 14433 P16858
Anti-Rat GAPDH GAPDH 24383 P04797
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Top anti-GAPDH Antibodies at antibodies-online.com

Showing 10 out of 855 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Dog Rabbit Un-conjugated WB 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain Sample Type: 3. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science 100 μL Log in to see 2 to 3 Days
$289.00
Details
Dog Rabbit Un-conjugated WB Host: Rabbit Target Name: GAPDH Sample Tissue: Mouse Pancreas Antibody Dilution: 1ug/ml WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: NCI-H226 cell lysate 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Mouse Un-conjugated EIA, IF, WB 0.1 mL Log in to see 6 to 8 Days
$412.50
Details
REACT_Broad Bean Rabbit Un-conjugated EIA, IF, IHC (p), WB 0.1 mg Log in to see 6 to 8 Days
$434.50
Details
Human Rabbit Un-conjugated FACS, IF, IHC (p), WB Western blot analysis of GAPDH Antibody (C-term R248) (ABIN392354) in A2058, A375, CEM cell line lysates (35 µg/lane). GAPDH (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Dog Goat Un-conjugated EIA, IHC (fro), WB 0.1 mg Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated IF (p), IHC (p), WB This image was generously provided by Loyda M. Meléndez at the University of Puerto Rico Medical Sciences Campus.A total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody  in 5% milk at 1:250 dilution overnight at 4°C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds. MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody  at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody -HRP ) at 1:5000 for 90 min at 37˚C. 100 μL Log in to see 3 to 7 Days
$307.45
Details
Mouse Rabbit Un-conjugated IF (p), IHC (p), WB Formalin-fixed and paraffin embedded rat kidney labeled with Anti GAPDH Polyclonal Antibody, Unconjugated (ABIN728818) at 1:200 followed by conjugation to the secondary antibody and DAB staining. 100 μL Log in to see 3 to 7 Days
$307.45
Details
Human Rabbit Un-conjugated IF, IHC (p), WB Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Western blot analysis of GAPDH Antibody (N-term) (ABIN392353) in A2058, A375, CEM cell line lysates (35 µg/lane). GAPDH (arrow) was detected using the purified polyclonal antibody. 400 μL Log in to see 10 to 11 Days
$324.50
Details

GAPDH Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-GAPDH Antibodies

  1. Human Polyclonal GAPDH Primary Antibody for IF, IHC (p) - ABIN392353 : Azam, Jouvet, Jilani, Vongsamphanh, Yang, Yang, Ramotar: Human glyceraldehyde-3-phosphate dehydrogenase plays a direct role in reactivating oxidized forms of the DNA repair enzyme APE1. in The Journal of biological chemistry 2008 (PubMed)
    Show all 9 references for 392353

  2. Broad Bean (Vicia faba) Polyclonal GAPDH Primary Antibody for EIA, IF - ABIN401403 : Sirover: New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. in Journal of cellular biochemistry 2005 (PubMed)
    Show all 7 references for 401403

  3. Dog (Canine) Polyclonal GAPDH Primary Antibody for EIA, IHC (fro) - ABIN374640 : Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy. in Circulation 2009 (PubMed)
    Show all 6 references for 374640

  4. Human Polyclonal GAPDH Primary Antibody for IF (p), IHC (p) - ABIN668886 : Shou, Lin, Liang, Li, Chen: Expression and prognosis of FOXO3a and HIF-1? in nasopharyngeal carcinoma. in Journal of cancer research and clinical oncology 2012 (PubMed)
    Show all 4 references for 668886

  5. Cow (Bovine) Polyclonal GAPDH Primary Antibody for IHC, WB - ABIN2778645 : Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes. in Human molecular genetics 2008 (PubMed)
    Show all 3 references for 2778645

  6. Human Polyclonal GAPDH Primary Antibody for FACS, IF - ABIN392354 : Lu, Suzuki, Lu, Suzuki: Involvement of glyceraldehyde-3-phosphate dehydrogenase in the X-Ray resistance of HeLa cells. in Bioscience, biotechnology, and biochemistry 2008 (PubMed)
    Show all 3 references for 392354

  7. Human Monoclonal GAPDH Primary Antibody for EIA, IF - ABIN492380 : Chuang, Hough, Senatorov: Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. in Annual review of pharmacology and toxicology 2005 (PubMed)
    Show all 2 references for 492380

  8. Mouse (Murine) Polyclonal GAPDH Primary Antibody for IF (p), IHC (p) - ABIN728818 : Tian, Zhang, Wang, Dai, Shen, Yang, Huang: The protective effect of hyperbaric oxygen and Ginkgo biloba extract on A?25-35-induced oxidative stress and neuronal apoptosis in rats. in Behavioural brain research 2013 (PubMed)
    Show all 2 references for 728818

More Antibodies against GAPDH Interaction Partners

Cow (Bovine) Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. GAPDH with CytOx could be the point of interest as it has already been shown that GAPDH protein damage results in a marked decrease in cellular ATP levels

  2. present in, and accounts for, glycolysis and glutamate (show GRIN2A Antibodies) accumulation into synaptic vesicles

  3. GAPDH phosphorylates the long intracellular loop of GABA(A) receptor alpha1 subunit at Ser (show SIGLEC1 Antibodies)-416 & Thr (show TRH Antibodies)-337. The neuronal membrane-attached GAPDH is a dual-purpose enzyme, a glycolytic dehydrogenase, & a receptor-associated kinase.

  4. Results show that expression of GAPD increased during the late fattening stage of of hanwoo steers and it is involved in metabolic pathways such as glycolysis and the TCA cycle.

Arabidopsis thaliana Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. GAPC levels play important roles in the overall cellular production of reductants, energy, and carbohydrate metabolites and that GAPC levels are directly correlated with seed oil accumulation.

  2. The addition of GSSG, and much more efficiently of S-nitrosoglutathione, was shown to inactivate the enzymes GapC1 and GapC2 from Arabidopsis thaliana, spinach, yeast and rabbit muscle. [GapC2]

Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. This suggests that RX624 might be useful as a drug against polyglutamine pathologies, and that is could be administered exogenously without affecting target cell physiology. This protective effect was validated by the similar effect of an anti-GAPDH specific antibody.

  2. GAPDH can interact with proteins participating in DNA repair, such as APE1 (show APEX1 Antibodies), PARP1 (show PARP1 Antibodies), HMGB1 (show HMGB1 Antibodies), and HMGB2 (show HMGB2 Antibodies). In this review, the functions of GAPDH associated with DNA repair are discussed in detail.

  3. Nitric oxide-induced GAPDH aggregation specifically induces mitochondrial dysfunction via permeability transition pore opening, leading to cell death.

  4. GAPDH may act as a chaperone in heme transfer to downstream areas

  5. NAD(+) inhibited both GAPDH aggregation and co-aggregation with GOSPEL (show RILPL1 Antibodies), a hitherto undescribed effect of the coenzyme against the consequences of oxidative stress.

  6. Monoclonal Antibodies DSHB-hGAPDH-2G7 and DSHB-hGAPDH-4B7 Against Human Glyceraldehyde-3-Phosphate Dehydrogenase.

  7. the present study suggests that GAPDH plays an important role in cancer metastasis by affecting EMT (show ITK Antibodies) through regulation of Sp1 (show PSG1 Antibodies)-mediated SNAIL (show SNAI1 Antibodies) expression.

  8. Knockdown of LAMP2A, a CMA-related protein, and TSG101 (show TSG101 Antibodies), an mA-related protein, significantly but only partially decreased the punctate accumulation of GAPDH-HT in AD293 cells and primary cultured rat cortical neurons.

  9. In conclusion, the data show that two GAPDH binders could be therapeutically relevant in the treatment of injuries stemming from hard oxidative stress.

  10. transient silencing of GAPDH reduces intracellular ROS (show ROS1 Antibodies) and facilitates increased autophagy, thereby reducing acute hypoxia and reoxygenation injury as well as the resulting apoptosis and necrosis.

Pig (Porcine) Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. The structure-bound sperm-specific enzymes, GAPDH-S and pyruvate kinase(PK-S), appeared only late in spermatogenesis simultaneously with the development of the structures to which they are bound.

Rabbit Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. The level of GAPDH-AP DNA adduct formation depends on oxidation of the protein SH-groups; disulfide bond reduction in GAPDH leads to the loss of its ability to form the adducts with AP DNA

  2. The strength, selectivity, reversibility, and redox sensitivity of heme binding to GAPDH are consistent with it performing heme sensing or heme chaperone-like functions in cells.

  3. Purified UCH-L1 (show UCHL1 Antibodies) and GAPDH were nitrated in vitro with peroxynitrite and the presence of nitrated proteins was confirmed by anti-3-nitrotyrosine Western blots.Subsequent validation with synthetic peptides was conducted for selected nitropeptides.

  4. dissociation of alpha-crystallin and GAPDH

  5. results strongly suggest that Tyr311 and Tyr317 nitration prohibits NAD(+) binding, leading to the loss of catalytic activity of GAPDH.

  6. In the presence of alpha-crystallin or GroEL (show GroEL Antibodies) the kinetic of GAPDH aggregation is changed from the diffusion-limited cluster-cluster aggregation to the reaction-limited cluster-cluster aggregation.

  7. The interactions described herein between the beta subunit (show POLG Antibodies) of PhK (show PHKA2 Antibodies) and GAPDH provide a possible mechanism for the direct linkage of glycogenolysis and glycolysis in skeletal muscle.

  8. FKBP36 (show FKBP6 Antibodies) diminishes GAPDH activity by direct interaction and down-regulation of GAPDH

Mouse (Murine) Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. These findings show that post-translational modification of GAPDH lysine residues regulates hepatic and systemic metabolism, revealing an unappreciated role for hepatic GAPDH in substrate selection and utilization.

  2. These results suggest that GAPDH is an ancient, evolutionarily conserved receptor that plays a key role in the Plg (show PLG Antibodies)-dependent regulation of macrophage recruitment in the inflammatory response to microbial aggression, thus pitting prokaryotic GAPDH against mammalian GAPDH, with both involved in a conserved role of Plg (show PLG Antibodies) activation on the surface of their respective cells, to conflicting ends.

  3. GAPDH-mediated expression regulation of sodium channel genes was associated with epilepsy and the anticonvulsant action of the ketogenic diet.

  4. Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC (show SCNN1A Antibodies) Activity

  5. Data indicate that lactoferrin (show LTF Antibodies) uptake across duodenum by glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

  6. astrocytic production of D-serine is modulated by glycolytic activity via interactions between GAPDH and SRR (show SRR Antibodies).

  7. Selective inhibition of the glycolytic pathway through GAPDH in chronic lymphocytic leukemia cells with nanoliposomal C6-ceramide could be an effective therapy for leukemia by targeting the Warburg effect.

  8. glyceraldehyde-3-phosphate dehydrogenase plays a critical role in determining the superior functions of female bone marrow-derived mesenchymal stem cells in cell therapy against pulmonary arterial hypertension.

  9. Data indicate that the HNO-nonsteroidal anti-inflammatory drugs (NSAIDs) prodrug inhibited cylcooxgenase-2 and glyceraldehyde 3-phosphate dehydrogenase activity and triggered significant sarcomere shortening on murine ventricular myocytes.

  10. Gapdh is a moonlighting protein that functions as a glycolytic enzyme and a transferrin receptor.

Xenopus laevis Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. The effect of metal ions on the activity of glyceraldehyde-3-phosphate dehydrogenase.

Zebrafish Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) interaction partners

  1. amino acid sequences were used to establish the 3D structures of the active site of glyceraldehyde-3-phosphate dehydrogenase and the phylogenetic relationships of the sardine and octopus enzymes

  2. The expression level of GAPDH was upregulated to a greater extent than those for myogenin (show MYOG Antibodies) and myostatin 1 under restricted feeding.

GAPDH Antigen Profile

Protein Summary

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with GAPDH

  • Glyceraldehyde 3 phosphate dehydrogenase 2 (Gapdh2) antibody
  • glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody
  • glyceraldehyde-3-phosphate dehydrogenase (gapdh) antibody
  • glyceraldehyde 3-phosphate dehydrogenase (Gapdh) antibody
  • glyceraldehyde 3-phosphate dehydrogenase (GAPC2) antibody
  • similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (GAPDH) antibody
  • glyceraldehyde-3-phosphate dehydrogenase, type I (gapDH) antibody
  • Glyceraldehyde 3 phosphate dehydrogenase 1 (Gapdh1) antibody
  • glyceraldehyde-3-phosphate dehydrogenase (Gapdh) antibody
  • glyceraldehyde-3-phosphate dehydrogenase-like (GAPDH) antibody
  • olfactory receptor, family 8, subfamily K, member 3-like (LOC614985) antibody
  • BEST:GH12586 antibody
  • cb609 antibody
  • CG8893 antibody
  • CG12055 antibody
  • Dmel\\CG8893 antibody
  • Dmel\\CG12055 antibody
  • G3PD antibody
  • G3PDH antibody
  • GA3PDH antibody
  • gadph antibody
  • GAP antibody
  • GAPC-2 antibody
  • gapd antibody
  • gapdh antibody
  • gapdh-1 antibody
  • Gapdh-2 antibody
  • GAPDH1 antibody
  • GAPDH2 antibody
  • Gapdh13F antibody
  • Gapdh43E antibody
  • GAPDH I antibody
  • GAPDHI antibody
  • GAPDH II antibody
  • GB14798 antibody
  • gh12586 antibody
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2 antibody
  • glyceraldehyde-3-phosphate dehydrogenase C2 antibody
  • KNC-NDS6 antibody
  • mg:bb02e05 antibody
  • T6J4.17 antibody
  • T6J4_17 antibody
  • wu:fb33a10 antibody
  • wu:ft80f05 antibody

Protein level used designations for GAPDH

CG8893-PA , CG8893-PB , CG8893-PC , G3-P dehydrogenase , GAPDH2 , Gapdh2-PA , Gapdh2-PB , Gapdh2-PC , glyceraldehyde 3 phosphate dehydrogenase 2 , glyceraldehyde 3-phosphate dehydrogenase , glyceraldehyde-3-phosphate dehydrogenase , glyceraldehyde phosphate dehydrogenase , peptidyl-cysteine S-nitrosylase GAPDH , glyceraldehyde-3-phosphate dehydrogenase, type I , glyceraldehyde-3-phosphate dehydrogenase-like , CG12055-PA , CG12055-PB , GAPDH1 , Gapdh1-PA , Gapdh1-PB , Glyceraldehyde-3-phosphate dehydrogenase1 , glyceraldehyde 3 phosphate dehydrogenase , glyceraldehyde 3 phosphate dehydrogenase 1 , glyceraldehyde 3 phosphate dehydrogenase1 , aging-associated gene 9 protein , 38 kDa BFA-dependent ADP-ribosylation substrate , BARS-38 , bb02e05

GENE ID SPECIES
32545 Drosophila melanogaster
281181 Bos taurus
448356 Xenopus (Silurana) tropicalis
410122 Apis mellifera
837904 Arabidopsis thaliana
100135863 Oncorhynchus mykiss
574353 Macaca mulatta
712631 Macaca mulatta
2734417 Bdellovibrio bacteriovorus HD100
8970424 Mycoplasma crocodyli MP145
100404960 Callithrix jacchus
100478741 Ailuropoda melanoleuca
35728 Drosophila melanogaster
374193 Gallus gallus
2597 Homo sapiens
396823 Sus scrofa
100009074 Oryctolagus cuniculus
14433 Mus musculus
24383 Rattus norvegicus
380259 Xenopus laevis
403755 Canis lupus familiaris
100860872 Capra hircus
493876 Felis catus
451783 Pan troglodytes
100135525 Cavia porcellus
317743 Danio rerio
443005 Ovis aries
100033897 Equus caballus
100172694 Pongo abelii
786101 Bos taurus
614985 Bos taurus
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