anti-Mdc1 (MDC1) Antibodies

The protein encoded by MDC1 contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence.

list all antibodies Gene Name GeneID UniProt
MDC1 240087 Q5PSV9
MDC1 309595 Q5U2M8
MDC1 9656 Q14676
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Top anti-MDC1 Antibodies at antibodies-online.com

Showing 10 out of 164 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Dog Rabbit Un-conjugated WB WB Suggested Anti-MDC1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: HepG2 cell lysate MDC1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ICC, IF, PLA, WB Detection of Human MDC1 by Western Blot. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody ABIN151859 used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies ABIN151860 and ABIN151861 used at 3.3 mcg/mg lysate for IP followed by WB using ABIN151458 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Proximity Ligation Assay: MDC1 Antibody [ABIN151859] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMC1 (NBP2-14858) and rabbit anti-human MDC1 (ABIN151859). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification. 100 μL Log in to see 7 to 9 Days
$486.94
Details
Human Rabbit Un-conjugated IHC (p), IP, IHC, WB Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody ABIN151860 used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies ABIN151860 and ABIN151861 used at 3.3 mcg/mg lysate for IP followed by WB using ABIN151859 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Immunohistochemistry-Paraffin: MDC1 Antibody [ABIN151860] - Human ovarian carcinoma. 100 μL Log in to see 7 to 9 Days
$486.94
Details
Chimpanzee Mouse Un-conjugated ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X. Western Blot analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody  at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa. 100 μg Log in to see 2 to 3 Days
$360.80
Details
Human Rabbit Un-conjugated ICC, IF, IHC (p), IP, IHC, WB Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody ABIN151851 used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies ABIN151860 and ABIN151861 used at 3.3 mcg/mg lysate for IP followed by WB using ABIN151860 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Immunohistochemistry-Paraffin: MDC1 Antibody [ABIN151861] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 0.1 mL Log in to see 7 to 9 Days
$486.94
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p) Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human colon shows strong nuclear and membranous positivity in glandular cells. Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & focal adhesions. 0.1 mL Log in to see 10 to 13 Days
$439.69
Details
Human Rabbit Un-conjugated IF (p), IHC (p) Antigen: 0.2 µg/100 µL  Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;  Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;  TMB staining;  Read the data in MicroplateReader by 450 Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti NFBD1/MDC1 Polyclonal Antibody, Unconjugated (ABIN739740) at 1:200 followed by conjugation to the secondary antibody and DAB staining 100 μL Log in to see 3 to 7 Days
$295.90
Details
Human Rabbit Un-conjugated IHC (p), WB IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1, antibody at 1:500 dilution. WB Image Sample (30 ug of whole cell lysate) A: HeLa nucleus 5% SDS PAGE antibody diluted at 1:1000 100 μL Log in to see 1 to 2 Days
$358.60
Details
Human Rabbit Un-conjugated EIA, WB   0.1 mg Log in to see 6 to 8 Days
$419.10
Details

MDC1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-MDC1 Antibodies

  1. Human Polyclonal MDC1 Primary Antibody for ICC, IF - ABIN151859 : Shi, Ma, Willers, Akhtar, Scott, Zhang, Powell, Zhang: Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. in The Journal of biological chemistry 2008 (PubMed)
    Show all 16 Pubmed References

  2. Human Polyclonal MDC1 Primary Antibody for IF, WB - ABIN2475546 : Dranove, Shanley, White: How fast are hospital prices really rising? in Medical care 1991 (PubMed)
    Show all 4 Pubmed References

  3. Human Polyclonal MDC1 Primary Antibody for ICC, IF - ABIN151861 : Mallette, Mattiroli, Cui, Young, Hendzel, Mer, Sixma, Richard: RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites. in The EMBO journal 2012 (PubMed)

  4. Human Polyclonal MDC1 Primary Antibody for IHC (p), IP - ABIN151860 : Floyd, Pacold, Huang, Clarke, Lam, Cannell, Bryson, Rameseder, Lee, Blake, Fydrych, Ho, Greenberger, Chen, Maffa, Del Rosario, Root, Carpenter, Hahn, Sabatini, Chen, White, Bradner, Yaffe: The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. in Nature 2013 (PubMed)

  5. Human Polyclonal MDC1 Primary Antibody for ICC, IF - ABIN4333285 : Mastrocola, Kim, Trinh, Rodenkirch, Tibbetts: The RNA-binding protein fused in sarcoma (FUS) functions downstream of poly(ADP-ribose) polymerase (PARP) in response to DNA damage. in The Journal of biological chemistry 2013 (PubMed)

More Antibodies against MDC1 Interaction Partners

Mouse (Murine) Mdc1 (MDC1) interaction partners

  1. MDC1 plays a fundamentally significant role in maintenance of genomic stability through a DNA damage response-independent pathway.

  2. MDC1 silencing enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in xenograft tumor growth inhibition.

  3. oligomerization of MDC1 plays an important role in DDR (show DDR1 Antibodies) and help understand the formation of proteins complexes at the sites of DNA damage.

  4. structural insight into MDC1-CHK2 (show CHEK2 Antibodies) interaction

  5. the DNA damage response pathway centered on MDC1 triggers epigenetic silencing of sex chromosomes in germ cells

  6. Data show that loss of Mof (show KAT8 Antibodies) leads to reduction of histone H4 (show HIST1H4H Antibodies) K16 (show KRT16 Antibodies) acetylation, cell cycle arrest, chromosome aberration, defects in DNA damage repair, and complete loss of Mdc1 response to DNA damage.

  7. Data show that ATM (show ATM Antibodies)-dependent resection at a subset of DSBs leads to ATR (show ATR Antibodies)-dependent Chk1 (show CHEK1 Antibodies) activation, and that 53BP1 (show TP53BP1 Antibodies)(-/-) and MDC1(-/-) cells manifest a checkpoint defect at high radiation doses.

  8. identification and description of a functional homologue of human MDC1/ NFBD1; in response to ionizing radiation it forms foci that co-localize with the MRE11 (show MRE11A Antibodies)-RAD50 (show RAD50 Antibodies)-NBS1 (show NBN Antibodies) (MRN) complex and factors such as gammaH2AX (show H2AFX Antibodies) and 53BP1 (show TP53BP1 Antibodies)

  9. MDC1, as a signal amplifier of the ATM (show ATM Antibodies) pathway, is vital in controlling proper DNA damage response and maintaining genomic stability.

  10. MDC1 knockdown affected the formation of telomere dysfunction-induced foci.

Human Mdc1 (MDC1) interaction partners

  1. ASF1a (show ASF1A Antibodies) promotes non-homologous end joining repair by facilitating phosphorylation of MDC1 by ATM (show ATM Antibodies) at double-strand breaks.

  2. the opposing activities of RNF4 (show RNF4 Antibodies) and ataxin-3 (show ATXN3 Antibodies) consolidate robust MDC1-dependent signaling and repair ofDNA double-strand break.

  3. NFBD1 protein is overexpressed in NPC (show NPC1 Antibodies) tissues and that silencing NFBD1 can inhibit cell growth, induce apoptosis, increase the production of intracellular ROS (show ROS1 Antibodies). NFBD1 knockdown also inhibits the tumorigenicity of NPC (show NPC1 Antibodies) cells in vivo.

  4. NFBD1 knockdown improves the chemosensitivity of NPC (show NPC1 Antibodies) cells by inhibiting cell growth and promoting apoptosis through the impairment of DNA damage repair, suggesting NFBD1 as a novel therapeutic target for NPC (show NPC1 Antibodies).

  5. knockdown of MCM2 or MCM6 could significantly inhibit foci forming of MDC1 in TE-1 nuclei in response to bleomycin-induced DNA damage (p < 0.001). This study indicates the direct interaction between MDC1 and MCMs in TE-1 nuclei.

  6. The interaction of MDC1 with RNF8 (show RNF8 Antibodies), but not with ATM (show ATM Antibodies) requires WRAP53beta, suggesting that WRAP53beta facilitates the former interaction without altering phosphorylation of MDC1 by ATM (show ATM Antibodies).

  7. MDC1 recruits TNKS1 (show TNKS Antibodies) and TNKS2 (show TNKS2 Antibodies) to DNA lesions.

  8. MDC1 silencing enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in xenograft tumor growth inhibition.

  9. we generated two HEP (show EPHB6 Antibodies)-2 cell lines with a stable knockdown of MDC1 or 53BP1 (show TP53BP1 Antibodies) with short hairpin RNA (shRNA), respectively, and investigated the effect of MDC1 and 53BP1 (show TP53BP1 Antibodies) on cell radiosensitivity

  10. During replicative senescence and stress-induced premature senescence, MDC1 is downregulated by upregulating miR (show MLXIP Antibodies)-22.

MDC1 Antigen Profile

Protein Summary

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Gene names and symbols associated with anti-Mdc1 (MDC1) Antibodies

  • mediator of DNA damage checkpoint 1 (Mdc1) antibody
  • mediator of DNA-damage checkpoint 1 (MDC1) antibody
  • 6820401C03 antibody
  • AA413496 antibody
  • mKIAA0170 antibody
  • NFBD1 antibody

Protein level used designations for anti-Mdc1 (MDC1) Antibodies

mediation of DNA damage checkpoint 1 , mediator of DNA damage checkpoint protein 1 , homologue to Drosophila photoreceptor protein calphotin , mediator of DNA damage checkpoint 1 , nuclear factor with BRCT domains 1

GENE ID SPECIES
240087 Mus musculus
309595 Rattus norvegicus
9656 Homo sapiens
474829 Canis lupus familiaris
100144453 Sus scrofa
511168 Bos taurus
471959 Pan troglodytes
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