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anti-N-Ethylmaleimide-Sensitive Factor (NSF) Antibodies

Required for vesicle-mediated transport. Additionally we are shipping NSF Proteins (7) and NSF Kits (5) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
NSF 18195 P46460
NSF 60355 Q9QUL6
NSF 4905 P46459
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Top anti-NSF Antibodies at antibodies-online.com

Showing 10 out of 92 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated WB 0.1 mL Log in to see 6 to 8 Days
$412.50
Details
Chicken Mouse Un-conjugated BCA, IF, WB Western blot analysis of NSF on a HeLa lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the anti- NSF antibody. Immunofluorescence staining of HeLa cells. 50 μg Log in to see 5 to 6 Days
$205.42
Details
Mouse Rabbit Un-conjugated WB NSF Antibody (C-term) (ABIN1881589) western blot analysis in mouse cerebellum tissue lysates (35 µg/lane). This demonstrates the NSF antibody detected the NSF protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Chicken Mouse Un-conjugated BI, IF, WB Western blot analysis of NSF on a HeLa lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the anti- NSF antibody. Immunofluoresence staining of HeLa cells. 150 μg Log in to see 5 to 6 Days
$371.97
Details
Chicken Rabbit Un-conjugated IHC, WB WB Suggested Anti-NSF Antibody Titration: 0.2-1 ug/mlPositive Control: Human brain Sample Type: 1. NT-2 cells2.  mouse brain extractsPrimary Antibody Dilution: 2ug/mlSecondary Antibody Dilution: 1: 20,000Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceLane 1: 60ug/laneLane 2: 80ug/laneImage Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science 100 μL Log in to see 2 to 3 Days
$289.00
Details
Rat Mouse Un-conjugated ICC, IP, IHC, ELISA, WB dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2) Indirect immunostaining of hippocampus neurons  (dilution 1 : 500) 100 μg Log in to see 5 to 6 Days
$444.68
Details
Rat Rabbit Un-conjugated IHC (p), IP, WB Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 8 to 10 Days
$451.00
Details
Human Rabbit Un-conjugated IHC, WB IHC on rat cerebellum (cryo sections) using Rabbit antibody to n-terminal of NSF (Vesicle fusing ATPase): whole serum (ABIN350527) at 1 : 2000 dilution. IHC on rat cerebellum (cryo sections) using Rabbit antibody to n-terminal of NSF (Vesicle fusing ATPase): whole serum (ABIN350527) at 1:2000 dilution. 100 μL Log in to see 7 to 8 Days
$454.67
Details
Human Rabbit Un-conjugated WB Western blot analysis of NSF expression in MCF7 (A), Raw264.7 (B), H9C2 (C) whole cell lysates. 200 μL Log in to see 9 Days
$487.50
Details
Mouse Rabbit Un-conjugated IHC (p), IP, WB Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 6 to 8 Days
$500.50
Details

NSF Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Rat (Rattus) ,
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Top referenced anti-NSF Antibodies

  1. Mouse (Murine) Polyclonal NSF Primary Antibody for WB - ABIN1881589 : Hamza, Zabetian, Tenesa, Laederach, Montimurro, Yearout, Kay, Doheny, Paschall, Pugh, Kusel, Collura, Roberts, Griffith, Samii, Scott, Nutt, Factor, Payami: Common genetic variation in the HLA region is associated with late-onset sporadic Parkinson's disease. in Nature genetics 2010 (PubMed)
    Show all 4 references for ABIN1881589

  2. Human Polyclonal NSF Primary Antibody for IHC, WB - ABIN350527 : Ballif, Carey, Sunyaev, Gygi: Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain. in Journal of proteome research 2008 (PubMed)
    Show all 3 references for ABIN350527

  3. Human Polyclonal NSF Primary Antibody for WB - ABIN372691 : Schweizer, Dresbach, DeBello, OConnor, Augustine, Betz: Regulation of neurotransmitter release kinetics by NSF. in Science (New York, N.Y.) 1998 (PubMed)
    Show all 3 references for ABIN372691

  4. Chicken Monoclonal NSF Primary Antibody for BI, IF - ABIN968797 : Noel, Ralph, Pickard, Williams, Molnar, Uney, Collingridge, Henley: Surface expression of AMPA receptors in hippocampal neurons is regulated by an NSF-dependent mechanism. in Neuron 1999 (PubMed)
    Show all 3 references for ABIN968797

  5. Chicken Monoclonal NSF Primary Antibody for BCA, IF - ABIN968796 : Otter-Nilsson, Hendriks, Pecheur-Huet, Hoekstra, Nilsson: Cytosolic ATPases, p97 and NSF, are sufficient to mediate rapid membrane fusion. in The EMBO journal 1999 (PubMed)
    Show all 3 references for ABIN968796

  6. Rat (Rattus) Monoclonal NSF Primary Antibody for ICC, IP - ABIN1742269 : Ohno, Ishihara, Terada, Kikuta, Sofue, Kawai, Serikawa, Sasa: Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling. in Biochemical and biophysical research communications 2009 (PubMed)

More Antibodies against NSF Interaction Partners

Xenopus laevis N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. NSF- and SNARE (show NAPA Antibodies)-mediated membrane fusion events are essential steps during Nuclear envelope formation downstream of Nup107 (show NUP107 Antibodies) recruitment, and upstream of membrane flattening and completion of nuclear pore complex assembly.

Mouse (Murine) N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. identify conserved sequences in the 3'UTR (show UTS2R Antibodies) of NSF as miR (show MLXIP Antibodies)-33 responsive elements and show that Nsf is specifically recruited to the RNA-induced silencing complex following induction of miR (show MLXIP Antibodies)-33

  2. Arr1 (show SAG Antibodies) and NSF interaction are necessary for both maintenance and modulation of normal photoreceptor synaptic regulation and NSF-RP2 interaction may play an important role in membrane protein trafficking in the photoreceptor.

  3. These findings identify new roles for N-ethylmaleimide-sensitive factor and protein kinase C epsilon (show PRKCE Antibodies) in regulating synaptic inhibition through downregulation of GABA(A) receptors

  4. interaction of AMPA (show GRIA3 Antibodies) receptors with N-ethylmaleimide-sensitive factor plays a critical role in incorporation of AMPA (show GRIA3 Antibodies) receptors into synapses and for their subsequent removal during cerebellar long term depression

  5. PICK1 (show PICK1 Antibodies) and NSF dynamically regulate the synaptic delivery of GluR2 (show GRIA2 Antibodies)-containing receptors during CARP (show ANKRD1 Antibodies) and thus regulate the calcium permeability of AMPA (show GRIA3 Antibodies) receptors at excitatory synapses.

  6. H(2)O(2) regulates exocytosis by inhibiting N-ethylmaleimide sensitive factor (NSF), a protein that regulates membrane fusion events necessary for exocytosis.

  7. important role for NSF activity in regulating the cell surface stability of GABA(A) receptors.

  8. dual regulation on hbeta2AR trafficking and signaling by NSF through direct binding to cargo receptor and its ATPase (show DNAH8 Antibodies) activity.

  9. These results indicate that phosphorylation of GluR2 (show GRIA2 Antibodies) S880 and NSF and PICK1 (show PICK1 Antibodies) binding to GluR2 (show GRIA2 Antibodies) dynamically regulate GluR2 (show GRIA2 Antibodies) recycling.

  10. Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide sensitive factor

Human N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. a processive helicase-like mechanism for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule.

  2. Single and multiple-marker analyses revealed a strong association between cocaine dependence and the NSF gene.

  3. Knockdown of TRX1 (show MLL Antibodies) increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis, and suppresses leukocyte

  4. NSF independent fusion of Salmonella-containing late phagosomes with early endosomes.

  5. analysis of human NSF possible binding mode to GABARAP (show GABARAP Antibodies) and GATE-16 (show GABARAPL2 Antibodies)

  6. binding of NSF to GluR2 (show GRIA2 Antibodies)-containing AMPARs in stabilizing these receptors in the synaptic membrane and impeding their regulated endocytosis.

  7. role of interaction with GluR2 (show GRIA2 Antibodies) in regulation of AMPA (show GRIA3 Antibodies) receptors by brain-derived neurotrophic factor (show BDNF Antibodies)

  8. Nitric oxide regulates exocytosis by S-nitrosylation of NSF.

  9. Expressing a biochemically defined NSF mutant that lacks ATPase (show DNAH8 Antibodies) activity results in a dramatic inhibition of stimulated mast cell exocytosis.

  10. NSF dissociates the VAMP-2 (show VAMP2 Antibodies).SNAP-25 (show SNAP25 Antibodies).syntaxin 13 (show STX12 Antibodies) complex, but has no effect on the Hrs-containing complex.

Cow (Bovine) N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. Report depletion of the ATPase (show DNAH8 Antibodies) NSF from Golgi membranes with hypo-S-nitrosylation of vasorelevant proteins in endothelial cells exposed to monocrotaline pyrrole.

Zebrafish N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. results establish a role for Nsf in the maintenance of synaptic contacts between hair cells and afferent neurons, mediated in part via the secretion of trophic signaling factors

  2. Nsf is essential in the formation of the nodes of Ranvier in the vertebrate nervous system.

  3. Activation of PKCgamma is necessary for the activity-dependent trafficking of AMPARs in embryonic zebrafish and is NMDA and SNARE-dependent and requires AMPARs to associate with both NSF and PICK1.

  4. This study analyses of nsf mutant zebrafish reveal an unexpected role for NSF in hypothalamic development, with mutant 5 days post-fertilization larvae exhibiting a stage-dependent loss of neuroendocrine transcripts.

NSF Antigen Profile

Protein Summary

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Gene names and symbols associated with NSF

  • N-ethylmaleimide-sensitive factor (NSF) antibody
  • N-ethylmaleimide-sensitive factor (nsf) antibody
  • N-ethylmaleimide-sensitive factor (BBOV_II007200) antibody
  • N-ethylmaleimide-sensitive factor (TGME49_118510) antibody
  • N-ethylmaleimide sensitive factor (NSF) antibody
  • N-ethylmaleimide sensitive factor (nsf) antibody
  • N-ethylmaleimide sensitive fusion protein (Nsf) antibody
  • N-ethylmaleimide-sensitive factor (Nsf) antibody
  • N-ethylmaleimide-sensitive factor a (nsfa) antibody
  • 18.m06598 antibody
  • AI316878 antibody
  • AU020090 antibody
  • AU067812 antibody
  • N-ethylmaleimide sensitive factor antibody
  • nsf antibody
  • si:bz18k17.1 antibody
  • SKD2 antibody
  • T1J1.4 antibody
  • T1J1_4 antibody
  • wu:fj33g11 antibody

Protein level used designations for NSF

vesicle-fusing ATPase , N-ethylmaleimide-sensitive factor , N-ethylmaleimide-sensitive fusion protein , NEM-sensitive fusion protein , vesicular-fusion protein NSF , vesicle-fusing ATPase-like , N-ethylmaleimide sensitive factor , suppressor of K(+) transport growth defect 2 , N-ethylmaleimide sensitive fusion protein , N-ethylmaleimide-sensitive factor-like protein

GENE ID SPECIES
373397 Strongylocentrotus purpuratus
454819 Pan troglodytes
780249 Xenopus (Silurana) tropicalis
5478472 Babesia bovis T2Bo
7901210 Toxoplasma gondii ME49
100024726 Monodelphis domestica
100049722 Xenopus laevis
100063690 Equus caballus
100174078 Pongo abelii
100180685 Ciona intestinalis
100472215 Ailuropoda melanoleuca
100519170 Sus scrofa
9310886 Arabidopsis lyrata subsp. lyrata
779070 Xenopus laevis
825830 Arabidopsis thaliana
18195 Mus musculus
60355 Rattus norvegicus
419972 Gallus gallus
4905 Homo sapiens
504457 Bos taurus
490921 Canis lupus familiaris
100624659 Sus scrofa
101120344 Ovis aries
368483 Danio rerio
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