anti-PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) Antibodies

Pre-mRNA splicing occurs in 2 sequential transesterification steps. Additionally we are shipping and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
PRPF8 287530  
PRPF8 10594 Q6P2Q9
PRPF8 192159 Q99PV0
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Top anti-PRPF8 Antibodies at antibodies-online.com

Showing 10 out of 26 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-PRPF8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate WB Suggested Anti-PRPF8  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500  Positive Control: Jurkat cell lysate  PRPF8 is supported by BioGPS gene expression data to be expressed in Jurkat 100 μL Log in to see 2 to 3 Days
$289.00
Details
Chicken Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
Details
Human Rabbit Un-conjugated IP, WB Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody at dilution of 1:100 (x40 lens). 100 μL Log in to see 16 Days
$460.63
Details
Human Mouse Un-conjugated DB, ICC, FACS, WB HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-PRPF8 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-PRPF8 antibody. 50 μg Log in to see 6 to 8 Days
$285.71
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22272] - FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 (Cat. No. NBP2-22272, left panel, NBP2-22273, middle panel and Cat. No. NBP2-22274, right panel) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB Western Blot: PRPF8 Antibody [NBP2-22272] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. 100 μL Log in to see 7 to 9 Days
$486.94
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, WB Immunoprecipitation: PRPF8 Antibody [NBP2-22273] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22273 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22274 For blotting immunoprecipitated PRPF8, NBP2-22273 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Western Blot: PRPF8 Antibody [NBP2-22273] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. 100 μL Log in to see 7 to 9 Days
$486.94
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, WB Immunoprecipitation: PRPF8 Antibody [NBP2-22274] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22274 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22273 For blotting immunoprecipitated PRPF8, NBP2-22274 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22274] - FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 (Cat. No. NBP2-22272, left panel ,NBP2-22273, middle panel and Cat. No. NBP2-22274, right panel) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB 100 μL Log in to see 7 to 9 Days
$486.94
Details
Human Mouse Un-conjugated DB, ICC, FACS, WB   50 μg Log in to see 7 to 9 Days
$493.17
Details
Human Rabbit Un-conjugated WB PRPF8 antibody used at 1 ug/ml to detect target protein. 50 μg Log in to see 2 to 3 Days
$473.93
Details
Human Mouse Un-conjugated ICC, FACS, WB   50 μg Log in to see 7 to 9 Days
$566.50
Details

PRPF8 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality
Rat (Rattus)
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Human
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Mouse (Murine)
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Top referenced anti-PRPF8 Antibodies

  1. Cow (Bovine) Polyclonal PRPF8 Primary Antibody for WB - ABIN2778986 : Pena, Liu, Bujnicki, Lührmann, Wahl: Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa. in Molecular cell 2007 (PubMed)

More Antibodies against PRPF8 Interaction Partners

Zebrafish PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) interaction partners

  1. Within early haematopoiesis, myeloid differentiation is impaired, suggesting Prpf8 is required for haematopoietic development.

Human PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) interaction partners

  1. We show that PRP31 (show PRPF31 Antibodies), a component of U4 snRNP (show LSM2 Antibodies), is modified with K63-linked ubiquitin chains by the PRP19 (show PRPF19 Antibodies) complex and deubiquitinated by USP15 (show USP15 Antibodies) and its substrate targeting factor SART3 (show SART3 Antibodies). USP15SART3 makes a complex with USP4 (show USP4 Antibodies) and this ternary complex serves as a platform to deubiquitinate PRP31 (show PRPF31 Antibodies) and PRP3 (show CLCA4 Antibodies)

  2. HSP90 (show HSP90 Antibodies)/R2TP chaperone system promotes the assembly of a key module of U5 snRNP (show LSM2 Antibodies) while assuring the quality control of PRPF8. The proteomics data further reveal new interactions between R2TP and the tuberous sclerosis complex, pointing to a potential link between growth signals and the assembly of key cellular machines.

  3. influenza A virus upregulates cellular PRPF8 gene expression through viral NS1 protein and influenza virus polymerase basic protein 1 to increase virus production.

  4. Our findings exemplify the regulatory potential of changes in the core spliceosome machinery, which may be relevant to slow-onset human genetic diseases linked to PRPF8 deficiency

  5. Most importantly between Prp8 and nucleotides at the exon-intron junction.

  6. A mutation in a splicing factor (show SLU7 Antibodies) PRPF8 that causes retinitis pigmentosa has a transcriptome-wide effect on mRNA splicing.

  7. Data suggest Enterovirus 3DPol (RNA-dependent RNA polymerase) enters nucleus via nuclear localization signal, targets pre-mRNA processing factor 8 (Prp8) to block pre-mRNA splicing/mRNA synthesis, and shuts off cellular transcription/translation.

  8. In the cytoplasm, Prp8 forms a precursor complex with U5 snRNA

  9. these data show how a Ski2 (show SKIV2L Antibodies)-like RNA helicase (show DDX46 Antibodies) Brr2 (show SNRNP200 Antibodies) can be reversibly inhibited by a protein cofactor Prp8 that directly competes with RNA substrate binding.

  10. This is the first report of marked intrafamilial variability associated with mutations in the PRPF8 gene, including incomplete penetrance. PRPF8 mutations should be suspected in patients with autosomal dominant retinitis pigmentosa.

Mouse (Murine) PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) interaction partners

  1. The mouse retinal pigment epithelium (RPE (show RPE Antibodies))is the primary cell affected by mutations in the RNA splicing factors (PRPF3 (show PRPF3 Antibodies), PRPF8, and PRPF31 (show PRPF31 Antibodies)), and these changes occur at an early age.

  2. found that PRP31 (show PRPF31 Antibodies) and PRPC8 as well as snRNAs are highly expressed in retinal cells

  3. The finding of similar degenerative changes in RPE (show RPE Antibodies) cells of all three mouse models suggests that the retinal pigment epithelium may be the primary cell type affected in the RNA splicing factor (show SLU7 Antibodies) forms of retinitis pigmentosa.

PRPF8 Antigen Profile

Protein Summary

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

Gene names and symbols associated with PRPF8

  • pre-mRNA processing factor 8 (Prpf8) antibody
  • pre-mRNA processing factor 8 (prpf8) antibody
  • PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) antibody
  • pre-mRNA processing factor 8 (PRPF8) antibody
  • AU019467 antibody
  • D11Bwg0410e antibody
  • DBF3/PRP8 antibody
  • HPRP8 antibody
  • id:ibd1257 antibody
  • ik:tdsubc_2a9 antibody
  • im:7141966 antibody
  • prp-8 antibody
  • Prp8 antibody
  • PRPC8 antibody
  • RP13 antibody
  • Sfprp8l antibody
  • SNRNP220 antibody
  • tdsubc_2a9 antibody
  • wu:fb37c02 antibody
  • wu:fb73e06 antibody
  • xx:tdsubc_2a9 antibody
  • zgc:56504 antibody

Protein level used designations for PRPF8

PRP8 pre-mRNA processing factor 8 homolog , pre-mRNA-processing-splicing factor 8 , 220 kDa U5 snRNP-specific protein , PRP8 homolog , U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p , apoptosis-regulated protein 1 , apoptosis-regulated protein 2 , p220 , precursor mRNA processing protein , splicing factor Prp8

GENE ID SPECIES
287530 Rattus norvegicus
393951 Danio rerio
379945 Xenopus laevis
417559 Gallus gallus
480651 Canis lupus familiaris
507371 Bos taurus
10594 Homo sapiens
192159 Mus musculus
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