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anti-Phosphoribosyl Pyrophosphate Amidotransferase (PPAT) Antibodies

The protein encoded by PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. Additionally we are shipping PPAT Proteins (5) and PPAT Kits (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
PPAT 5471 Q06203
PPAT 117544 P35433
PPAT 231327  
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Top anti-PPAT Antibodies at antibodies-online.com

Showing 10 out of 64 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated IHC, WB Sample Type: 1. Human Skin Fibroblasts (100ug)2. HepG2 cells (20ug)3. HEK273 cells (20ug)4. HeLa skin cells(20ug)5. Hamster CHO K1 cells (20ug)Primary Dilution: 1:5000Secondary Antibody: Clean-Blot IP detection Reagent and KitSecondary Dilution: 1:2000Image Submitted By: V. Skopova, M. ZikanovaInstitute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in Prague 100 μL Log in to see 2 to 3 Days
$289.00
Details
Dog Rabbit Un-conjugated WB WB Suggested Anti-PPAT Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysate Host: Rabbit  Target Name: PPAT  Sample Tissue: Human Fetal Heart  Antibody Dilution: 1.0 µg/mL 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated EIA, WB 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated WB Western blot analysis of anti-PPAT Antibody (Center) (ABIN389228) in HepG2 cell line lysates (35 µg/lane).PPAT(arrow) was detected using the purified polyclonal antibody (1:60dilution). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Mouse Un-conjugated WB HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. 100 μL Log in to see 2 to 3 Days
$423.50
Details
Human Mouse Un-conjugated FACS, WB HEK293T cells transfected with either RC201144 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPAT antibody (ABIN2455709), and then analyzed by flow cytometry. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. 100 μL Log in to see 2 to 3 Days
$423.50
Details
Human Mouse Un-conjugated IHC, WB HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PPAT mouse monoclonal antibody. (ABIN2455804) 100 μL Log in to see 2 to 3 Days
$482.90
Details
Human Mouse Un-conjugated IHC (p), WB Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) 100 μL Log in to see 8 to 10 Days
$595.83
Details
Human Rabbit Un-conjugated ICC, IF, WB WB Image Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE antibody diluted at 1:1000 ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PPAT, antibody at 1:500 dilution. 100 μL Log in to see 2 to 3 Days
$358.60
Details
Human Mouse Un-conjugated FACS, WB 100 μL Log in to see 8 to 10 Days
$493.17
Details

PPAT Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-PPAT Antibodies

  1. Human Polyclonal PPAT Primary Antibody for EIA, WB - ABIN357903 : Bera, Chen, Smith, Zalkin: Interdomain signaling in glutamine phosphoribosylpyrophosphate amidotransferase. in The Journal of biological chemistry 2000 (PubMed)
    Show all 2 references for ABIN357903

More Antibodies against PPAT Interaction Partners

Fruit Fly (Drosophila melanogaster) Phosphoribosyl Pyrophosphate Amidotransferase (PPAT) interaction partners

  1. DREF (show ZBED1 Antibodies) binds directly upstream of Prat and the DRE (show SUFUH Antibodies) sites associated with its activity are necessary for Prat expression

  2. Data show the presence of apoptosis at the cellular level in both ade2 (show PAICS Antibodies) and Prat mutants, and the upregulated gene HtrA2 (show HTRA2 Antibodies), which encodes an apoptosis effector and is thus a candidate for initiating apoptosis in response to purine depletion.

  3. whereas Mod (show MOD(MDG4) Antibodies)(Prat:bw)3-5 suppresses Prat in all stages and tissues examined for both sexes.

  4. Evidence suggests that Prat originated by gene duplication by retrotransposition of Prat2.

  5. Regulation of expression of prat in the adult eye was studied.

  6. Use of Prat and Prat2 double RNA interference causes more severe pupal lethal phenotypes and suggests that Prat and Prat2 have partially additive functions during Drosophila metamorphosis.

  7. Data show that the requirement for both Prat and Prat2 in Drosophila is not simply due to differences in protein function. Both genes have developed nonoverlapping patterns of expression, which correlate with their respective loss-of-function phenotypes.

Human Phosphoribosyl Pyrophosphate Amidotransferase (PPAT) interaction partners

  1. purine biosynthetic pathway enzymes PPAT and PAICS, as well as pyruvate kinase activity are increased in lung cancer

PPAT Antigen Profile

Protein Summary

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

Gene names and symbols associated with PPAT

  • Phosphoribosylamidotransferase (Prat) antibody
  • phosphoribosyl pyrophosphate amidotransferase (LOC100220341) antibody
  • phosphoribosyl pyrophosphate amidotransferase (LOC100223932) antibody
  • phosphoribosyl pyrophosphate amidotransferase (LOC100348590) antibody
  • phosphoribosyl pyrophosphate amidotransferase (PPAT) antibody
  • phosphoribosyl pyrophosphate amidotransferase (ppat) antibody
  • phosphoribosyl pyrophosphate amidotransferase (Ppat) antibody
  • 5730454C12Rik antibody
  • AA408689 antibody
  • AA675351 antibody
  • AI507113 antibody
  • Atase antibody
  • C79945 antibody
  • CG2867 antibody
  • Dmel\\CG2867 antibody
  • DmPrat antibody
  • GPAT antibody
  • PRAT antibody

Protein level used designations for PPAT

CG2867-PA , CG2867-PB , Prat-PA , Prat-PB , phosphoribosylamidotransferase , transcript D , phosphoribosyl pyrophosphate amidotransferase , amidophosphoribosyltransferase-like , amidophosphoribosyltransferase , glutamine PRPP amidotransferase , glutamine phosphoribosylpyrophosphatate amidotransferase , glutamine phosphoribosylpyrophosphate amidotransferase , GPAT , ATase

GENE ID SPECIES
40935 Drosophila melanogaster
100220341 Taeniopygia guttata
100223932 Taeniopygia guttata
100348590 Oryctolagus cuniculus
100415443 Callithrix jacchus
398956 Xenopus laevis
5471 Homo sapiens
611407 Canis lupus familiaris
509652 Bos taurus
117544 Rattus norvegicus
231327 Mus musculus
422743 Gallus gallus
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