rBmKTX

Details for Product No. ABIN1001605, Supplier: Log in to see
Reactivity
Scorpion
Host
Escherichia coli (E. coli)
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Sequence VGINVKCKHS GQCLKPCKDA GMRFGKCING KCDCTPK.
Specificity Voltage-gated channel (Kv1.3) blocker. rBmKTX blocks Kv1.3 channels with IC50 of 019 nM.
Characteristics Source: Mesobuthus Martensii (Buthus martensii).
Molecular formula: C169H274N52O47S7.
Disulfide bonds between Cys7-Cys27, Cys13-Cys32, and Cys17-Cys34.
Purity > 98 %
Background BmKTX inhibits Kv1.3 channels heterologously expressed in HEK-293 cells. Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel currents were elicited by depolarizing the voltage steps of 200 ms from the holding potential -80 mV to +50 mV.
Molecular Weight 3962
Application Notes 0.1-1nM
Comment

K+ channel toxin -KTx 3.6

Restrictions For Research Use only
Format Lyophilized
Reconstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.262ml will provide a stock solution of 10 M.
Handling Advice Soluble in Water.
Supplier Images
 image for rBmKTX (ABIN1001605) Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel cu...
Product cited in: Renisio, Romi-Lebrun, Blanc, Bornet, Nakajima, Darbon: "Solution structure of BmKTX, a K+ blocker toxin from the Chinese scorpion Buthus Martensi." in: Proteins, Vol. 38, Issue 1, pp. 70-8, 2000 (PubMed).

Dai, Wu, Gu, Lan, Ling, Chi: "Genomic organization of three novel toxins from the scorpion Buthus martensi Karsch that are active on potassium channels." in: The Biochemical journal, Vol. 346 Pt 3, pp. 805-9, 2000 (PubMed).

Romi-Lebrun, Lebrun, Martin-Eauclaire, Ishiguro, Escoubas, Wu, Hisada, Pongs, Nakajima: "Purification, characterization, and synthesis of three novel toxins from the Chinese scorpion Buthus martensi, which act on K+ channels." in: Biochemistry, Vol. 36, Issue 44, pp. 13473-82, 1997 (PubMed).