rCharybdotoxin

Details for Product No. ABIN1001607, Supplier: Log in to see
Reactivity
Scorpion
Host
Escherichia coli (E. coli)
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Sequence QFTNVSCTTS KECWSVCQRL HNTSRGKCMN KKCRCYS.
Specificity Charybdotoxin is a potent selective inhibitor of high conductance (maxi-K), different medium and small conductance Ca2+-activated K+ channels, as well as a voltage-dependent K+ channel (Kv1.3).
Characteristics Source: Leiurus quinquestriatus hebraeus (Yellow scorpion).
Molecular formula: C176H280N58O55S7.
Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.
Purity > 98 %
Background Inhibition of mSlo channels heterologously expressed in HEK293 cells by Charybdotoxin. mSlo channel currents, before and during application of 100nM rCharybdotoxin. The currents were activated by a 50 ms voltage step to +100 mV from a prepulse of -180 mV with intracellular 10M Ca2+.
Molecular Weight 4313
Application Notes 10-100 nM
Comment

K+ channel toxin -KTx 1.1, ChTX, ChTX-Lq1, ChTx-a.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.160ml will provide a stock solution of 10 M.
Handling Advice Soluble in Water.
Supplier Images
 image for rCharybdotoxin (ABIN1001607) mSlo channel currents, before and during application of 100nM rCharybdotoxin. The cur...
Background publications Meera, Wallner, Toro: "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5562-7, 2000 (PubMed).

Product cited in: Vita, Bontems, Bouet, Tauc, Poujeol, Vatanpour, Harvey, Menez, Toma: "Synthesis of charybdotoxin and of two N-terminal truncated analogues. Structural and functional characterisation." in: European journal of biochemistry / FEBS, Vol. 217, Issue 1, pp. 157-69, 1993 (PubMed).

Gimenez-Gallego, Navia, Reuben, Katz, Kaczorowski, Garcia: "Purification, sequence, and model structure of charybdotoxin, a potent selective inhibitor of calcium-activated potassium channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 10, pp. 3329-33, 1988 (PubMed).

Smith, Phillips, Miller: "Purification of charybdotoxin, a specific inhibitor of the high-conductance Ca2+-activated K+ channel." in: The Journal of biological chemistry, Vol. 261, Issue 31, pp. 14607-13, 1986 (PubMed).

Miller, Moczydlowski, Latorre, Phillips: "Charybdotoxin, a protein inhibitor of single Ca2+-activated K+ channels from mammalian skeletal muscle." in: Nature, Vol. 313, Issue 6000, pp. 316-8, 1985 (PubMed).