rBeKm-1

Details for Product No. ABIN1001634, Supplier: Log in to see
Reactivity
Scorpion
Host
Escherichia coli (E. coli)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Sequence RPTDIKCSES YQCFPVCKSR FGKTNGRCVN GFCDCF.
Specificity BeKm-1 inhibits ERG1 K+ channel currents and has minimal effect on ELK1 K+ channels.
Characteristics Source: Buthus eupeus (Lesser Asian scorpion).
Molecular formula: C174H267N51O52S6.
Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.
Purity > 98 %
Background BeKm-1 inhibits Kv11.1 (hERG) channels expressed in HEK-293 cells. hERG channel currents, before and after application of 1nM rBeKm-1. hERG channel currents were elicited by stepping the potential to -50 mV to +50 mV in 10 mV steps (1-s test potentials) from a holding potential of -80 mV for activation.
Molecular Weight 4092
Application Notes 2-10 nM
Comment

K+ channel toxin -KTx 2.1

Restrictions For Research Use only
Format Lyophilized
Reconstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.222ml will provide a stock solution of 10 M.
Handling Advice Soluble in Water.
Supplier Images
 image for rBeKm-1 (ABIN1001634) hERG channel currents, before and after application of 1nM rBeKm-1. hERG channel curr...
Product cited in: Korolkova, Kozlov, Lipkin, Pluzhnikov, Hadley, Filippov, Brown, Angelo, Strøbaek, Jespersen, Olesen, Jensen, Grishin: "An ERG channel inhibitor from the scorpion Buthus eupeus." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 9868-76, 2001 (PubMed).

Filippov, Kozlov, Pluzhnikov, Grishin, Brown: "M-type K+ current inhibition by a toxin fron the scorpion Buthus eupeus." in: FEBS letters, Vol. 384, Issue 3, pp. 277-80, 1996 (PubMed).