rScyllatoxin

Details for Product No. ABIN1001639, Supplier: Log in to see
Reactivity
Scorpion
Host
Escherichia coli (E. coli)
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Sequence AFCNLRMCQL SCRSLGLLGK CIGDKCECVK H.
Specificity Scyllatoxin inhibits small conductance Ca2+-activated K+ channels (SK) 1-3.
Characteristics Source: Leiurus quinquestriatus hebraeus (Yellow scorpion).
Molecular formula: C142H237N45O39S7.
Disulfide bonds between Cys3-Cys21, Cys8-Cys26, and Cys12-Cys28.
Purity > 98 %
Background Scyllatoxin inhibits SKCa3 channels heterologously expressed in HEK-293 cells. SKCa3 channel currents, before and after application of 100nM rScyllatoxin. The SKCa currents were elicited by 1µM internal calcium and 200 ms voltage ramps from -140 to +40 mV at the holding potential of 0 mV.
Molecular Weight 3424
Application Notes 20-200 nM
Comment

K+ channel toxin -KTx 5.1, Leiurotoxin I, LeTxI, ScyTx

Restrictions For Research Use only
Format Lyophilized
Reconstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.460ml will provide a stock solution of 10 M.
Handling Advice Soluble in Water.
Supplier Images
 image for rScyllatoxin (ABIN1001639) SKCa3 channel currents, before and after application of 100nM rScyllatoxin. The SKCa ...
Product cited in: Rodríguez de la Vega, Possani: "Current views on scorpion toxins specific for K+-channels." in: Toxicon : official journal of the International Society on Toxinology, Vol. 43, Issue 8, pp. 865-75, 2004 (PubMed).

Pedarzani, Kulik, Muller, Ballanyi, Stocker: "Molecular determinants of Ca2+-dependent K+ channel function in rat dorsal vagal neurones." in: The Journal of physiology, Vol. 527 Pt 2, pp. 283-90, 2000 (PubMed).

Strøbaek, Jørgensen, Christophersen, Ahring, Olesen: "Pharmacological characterization of small-conductance Ca(2+)-activated K(+) channels stably expressed in HEK 293 cells." in: British journal of pharmacology, Vol. 129, Issue 5, pp. 991-9, 2000 (PubMed).

Wadsworth, Doorty, Strong: "Comparable 30-kDa apamin binding polypeptides may fulfill equivalent roles within putative subtypes of small conductance Ca(2+)-activated K+ channels." in: The Journal of biological chemistry, Vol. 269, Issue 27, pp. 18053-61, 1994 (PubMed).

Grissmer, Lewis, Cahalan: "Ca(2+)-activated K+ channels in human leukemic T cells." in: The Journal of general physiology, Vol. 99, Issue 1, pp. 63-84, 1992 (PubMed).

Auguste, Hugues, Mourre, Moinier, Tartar, Lazdunski: "Scyllatoxin, a blocker of Ca(2+)-activated K+ channels: structure-function relationships and brain localization of the binding sites." in: Biochemistry, Vol. 31, Issue 3, pp. 648-54, 1992 (PubMed).

Goh, Kelly, Pennefather, Chicchi, Cascieri, Garcia, Kaczorowski: "Effect of charybdotoxin and leiurotoxin I on potassium currents in bullfrog sympathetic ganglion and hippocampal neurons." in: Brain research, Vol. 591, Issue 1, pp. 165-70, 1992 (PubMed).

Auguste, Hugues, Gravé, Gesquière, Maes, Tartar, Romey, Schweitz, Lazdunski: "Leiurotoxin I (scyllatoxin), a peptide ligand for Ca2(+)-activated K+ channels. Chemical synthesis, radiolabeling, and receptor characterization." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4753-9, 1990 (PubMed).

Chicchi, Gimenez-Gallego, Ber, Garcia, Winquist, Cascieri: "Purification and characterization of a unique, potent inhibitor of apamin binding from Leiurus quinquestriatus hebraeus venom." in: The Journal of biological chemistry, Vol. 263, Issue 21, pp. 10192-7, 1988 (PubMed).