GFP-Booster (Atto 488)

Details for Product No. ABIN1082212
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Target Name (Antigen)
Aequorea victoria
Atto 488
Immunofluorescence (IF), Fluorescence Microscopy (FM)
Pubmed 21 references available
Quantity 50 μL
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Purpose With our Booster you reactivate, boost, stabilizate the signals of your fusion proteins.
Specificity GFP-Booster efficiently detects and labels most common GFP derivates. No binding to red fluorescent proteins derived from DsRed can be detected.
  • Enhance, stabilize and reactivate your fl uorescent proteins
  • GFP-Booster highly specifi c for GFP fusion proteins (and derivatives thereof e.g. YFP or Venus)
  • Coupled to bright and photostable chemical dyes from ATTO-TEC
Components GFP-Trap® coupled to fluorescent dye ATTO 488
Alternative Name GFP
Background Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics in living cells. However, photo stability and quantum efficiency of GFP are not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples. In addition, many cell biological methods such as BrdU-staining, EdU-Click-iT™ treatment or Fluorescent In Situ Hybridization result in disruption of the GFP signal.The GFP-Booster_Atto488, a specific GFP-binding protein coupled to the fluorescent dye ATTO 488, reactivates, boosts and stabilizes your GFP signal.
Research Area Tags/Labels
Application Notes For the immunofluorescence staining of GFP-fusion proteins in fixed cells

ATTO 488:
Excitation range 480 - 510 nm (λabs= 501 nm)
Emission range 520 - 560 nm (λfl= 523 nm)

Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC.

Assay Procedure
  • 1. Fixation: 4% paraformaldehyde (PFA) or 1:10 formalin (37% formaldehyde, 10-15% MetOH) in PBS, 10 min., RT.
  • 2. Wash 3x with PBS containing 0.1% Tween 20 (PBST). Critical: do not let coverslips “dry”.
  • 3. Permeabilisation: PBS containing 0.5% Triton X-100, 5 min., RT. Alternatively permeabilise by incubating in 100% methanol for 5min at -20°C.
  • 4. Wash 2x with PBST.
  • 5. Blocking: 4% BSA in PBST, 10 min, RT.
  • 6. GFP-Booster incubation: dilute GFP-Booster 1:200 in blocking buffer and incubate 1 h, RT. Note: For multiplexing protocols you can combine GFP-Booster with any other antibody.
  • 7. Wash 3x 5-10 min in PBST.
  • 8. If required counterstain with DNA fluorescent dyes, e.g. DAPI.
  • 9. Before mounting coverslips can be very briefly rinsed in water to prevent salt crystals to form.
  • 10. Mount in VectaShield (Vector Labs) or other mounting media with anti-fading agents and seal mounted coverslips with clear nail polish. Please note: Optimal dilutions/ concentrations should be determined by the end user
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS, 0.01% Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze. Protect from light.
Storage 4 °C
Expiry Date 6 months
Supplier Images
Image no. 1 for GFP-Booster (Atto 488) (ABIN1082212) Enhancement of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click-i...
Immunofluorescence (IF) image for GFP-Booster (Atto 488) (ABIN1082212) Visualization of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click...
Image no. 3 for GFP-Booster (Atto 488) (ABIN1082212) Enhancement of GFP signal with GFP-Booster_Atto488. Comparison of signal intensity of...
Product cited in: Richens, Barros, Lucas et al.: "The Drosophila Pericentrin-like-protein (PLP) cooperates with Cnn to maintain the integrity of the outer PCM." in: Biology open, Vol. 4, Issue 8, pp. 1052-61, 2015 (PubMed).

Vleugel, Hoek, Tromer et al.: "Dissecting the roles of human BUB1 in the spindle assembly checkpoint." in: Journal of cell science, Vol. 128, Issue 16, pp. 2975-82, 2015 (PubMed).

Riemer, Bontems, Krishnakumar et al.: "A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish." in: Gene expression patterns : GEP, Vol. 18, Issue 1-2, pp. 44-52, 2015 (PubMed).

Seybold, Elserafy, Rüthnick et al.: "Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope." in: The Journal of cell biology, Vol. 209, Issue 6, pp. 843-61, 2015 (PubMed).

Qin, Wolf, Liu et al.: "DNA methylation requires a DNMT1 ubiquitin interacting motif (UIM) and histone ubiquitination." in: Cell research, Vol. 25, Issue 8, pp. 911-29, 2015 (PubMed).

Vietri, Schink, Campsteijn et al.: "Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing." in: Nature, Vol. 522, Issue 7555, pp. 231-5, 2015 (PubMed).

Vleugel, Omerzu, Groenewold et al.: "Sequential multisite phospho-regulation of KNL1-BUB3 interfaces at mitotic kinetochores." in: Molecular cell, Vol. 57, Issue 5, pp. 824-35, 2015 (PubMed).

Eikenes, Malerød, Christensen et al.: "ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo." in: PLoS genetics, Vol. 11, Issue 1, pp. e1004904, 2015 (PubMed).

Wong, Chen, Lin et al.: "A TRPV channel in Drosophila motor neurons regulates presynaptic resting Ca2+ levels, synapse growth, and synaptic transmission." in: Neuron, Vol. 84, Issue 4, pp. 764-77, 2014 (PubMed).

Agircan, Schiebel: "Sensors at centrosomes reveal determinants of local separase activity." in: PLoS genetics, Vol. 10, Issue 10, pp. e1004672, 2014 (PubMed).

Oliveira, Kotadia, Tavares et al.: "Centromere-Independent Accumulation of Cohesin at Ectopic Heterochromatin Sites Induces Chromosome Stretching during Anaphase." in: PLoS biology, Vol. 12, Issue 10, pp. e1001962, 2014 (PubMed).

Bourge, Fort, Soler et al.: "A pulse-chase strategy combining click-EdU and photoconvertible fluorescent reporter: tracking Golgi protein dynamics during the cell cycle." in: The New phytologist, 2014 (PubMed).

Bluteau, Zhuang, Amann et al.: "Targeted disruption of the intracellular domain of receptor FgfrL1 in mice." in: PLoS ONE, Vol. 9, Issue 8, pp. e105210, 2014 (PubMed).

Winterflood, Ewers: "Single-Molecule Localization Microscopy using mCherry." in: Chemphyschem : a European journal of chemical physics and physical chemistry, Vol. 15, Issue 16, pp. 3447-51, 2014 (PubMed).

Bleck, Itano, Johnson et al.: "Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 33, pp. 12211-6, 2014 (PubMed).

Britton, Dernoncourt, Delteil et al.: "DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal." in: Nucleic acids research, Vol. 42, Issue 14, pp. 9047-62, 2014 (PubMed).

Tarancón Díez, Bönsch, Malkusch et al.: "Coordinate-based co-localization-mediated analysis of arrestin clustering upon stimulation of the C-C chemokine receptor 5 with RANTES/CCL5 analogues." in: Histochemistry and cell biology, Vol. 142, Issue 1, pp. 69-77, 2014 (PubMed).

Vázquez-Novelle, Sansregret, Dick et al.: "Cdk1 inactivation terminates mitotic checkpoint surveillance and stabilizes kinetochore attachments in anaphase." in: Current biology : CB, Vol. 24, Issue 6, pp. 638-45, 2014 (PubMed).

Kruse, Larsen, Sedgwick et al.: "A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment." in: EMBO reports, Vol. 15, Issue 3, pp. 282-90, 2014 (PubMed).

Biermann, Sokoll, Klueva et al.: "Imaging of molecular surface dynamics in brain slices using single-particle tracking." in: Nature communications, Vol. 5, pp. 3024, 2014 (PubMed).

Winterhoff, Junemann, Nordholz et al.: "The Diaphanous-related formin dDia1 is required for highly directional phototaxis and formation of properly sized fruiting bodies in Dictyostelium." in: European journal of cell biology, Vol. 93, Issue 5-6, pp. 212-24, 2014 (PubMed).

Catalog No. ABIN1082212
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