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Immunofluorescence (IF), Immunhistochemie (IHC)
|5 references available|
|Quantity||50 µg (1 mg/ml)|
|Price||210.54 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Description||GFP-Trap® coupled to fluorescent dye ATTO 488|
|Specificity||GFP-Booster efficiently detects and labels most common GFP derivates. No binding to red fluorescent proteins derived from DsRed can be detected.|
Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC.
1. Fixation: 4% paraformaldehyde (PFA) or 1:10 formalin (37% formaldehyde, 10-15% MetOH) in PBS, 10 min., RT.
2. Wash 3x with PBS containing 0.1% Tween 20 (PBST). Critical: do not let coverslips “dry”.
3. Permeabilisation: PBS containing 0.5% Triton X-100, 5 min., RT. Alternatively permeabilise by incubating in 100% methanol for 5min at -20°C.
4. Wash 2x with PBST.
5. Blocking: 4% BSA in PBST, 10 min, RT.
6. GFP-Booster incubation: dilute GFP-Booster 1:200 in blocking buffer and incubate 1 h, RT. Note: For multiplexing protocols you can combine GFP-Booster with any other antibody.
7. Wash 3x 5-10 min in PBST.
8. If required counterstain with DNA fluorescent dyes, e.g. DAPI.
9. Before mounting coverslips can be very briefly rinsed in water to prevent salt crystals to form.
10. Mount in VectaShield (Vector Labs) or other mounting media with anti-fading agents and seal mounted coverslips with clear nail polish. Please note: Optimal dilutions/ concentrations should be determined by the end user
|Application Notes||GFP-Booster now enables you to use your existing GFP expression constructs and cell lines also for super-resolution microscopy.|
|Preservative||0.01% Sodium azide|
|Storage||Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze. Protect from light.|
|Restrictions||For Research Use only|
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