Phospho Btk Flex Set (Bead D5)

Details for Product No. ABIN1379714, Supplier: Log in to see
Target Name (Antigen)
  • BTK
  • atk
  • bpk
  • xla
  • imd1
  • agmx1
  • psctk1
  • AGMX1
  • AT
  • ATK
  • BPK
  • IMD1
  • PSCTK1
  • XLA
  • AI528679
  • xid
  • Bruton agammaglobulinemia tyrosine kinase
  • BTK
  • btk
  • Btk
Epitope
pTyr551
Reactivity
Human
Application
Immunoassay (IA), Flow Cytometry (FACS)
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Brand BD™ Cytometric Bead Array,BD™
Characteristics The BD™ CBA Phospho Btk (Y551) Flex Set is a bead-based immunoassay capable of measuring human Bruton's tyrosine kinase (Btk), a non-receptor tyrosine kinase, which has been tyrosine-phosphorylated at Tyr-551 in denatured cell lysate samples. Human reactivity was determined by testing cell lysates with the BD CBA Phospho Btk (Y551) Flex Set. There are several publications evaluating the biology and function of Btk. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
The Phospho Btk (Y551) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 ml microfuge tube for reconstitution. Reconstitute the standard with 0.1 ml Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/ml and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use
Components Capture beads, detection reagent, standards
Alternative Name Btk
Comment

Bead Position: D5

Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
Buffer Aqueous buffered solution containing fetal bovine serum and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho Btk (Y551) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
Supplier Images
ELISA image for Phospho Btk Flex Set (Bead D5) (ABIN1379714) Phospho Btk Flex Set (Bead D5)
Product cited in: Rawlings, Scharenberg, Park, Wahl, Lin, Kato, Fluckiger, Witte, Kinet: "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases." in: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 822-5, 1996 (PubMed).