Mouse TrueBlot® Anti-Mouse Ig Biotin

Details for Product No. ABIN1589967, Supplier: Log in to see
Reactivity
Mouse (Murine)
Host
Rat
Clonality
Monoclonal
Conjugate
Biotin
Application
Immunoprecipitation (IP), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Brand TrueBlot®
Characteristics Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blotting with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. The Biotin Mouse TrueBlot® ULTRA is the biotinylated format of Mouse IgG TrueBlot® ULTRA. It can be used as a secondary antibody, followed by the use of avidin-peroxidase conjugated (A003-03).
Conjugation Name: Biotin TrueBlot® ULTRA
Components Mouse TrueBlot®: Anti-Mouse Ig Biotin
Research Area Immunology, Secondary Antibodies
Application Notes Western Blot Dilution: 1:2000
Comment

Biotin Mouse TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Biotin Mouse TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Biotin Mouse TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Note: There are two key procedural considerations:
1. When using any TrueBlot® reagent, ensure there is complete reduction of the lysate.
2. Use milk powder for complete and effective blocking of the western blot.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.09 % (w/v) Sodium Azide and proprietary stabiliser
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze. Dilute only prior to immediate use.
Storage 4 °C
Storage Comment Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
Expiry Date 6 months
Supplier Images
 image for Mouse TrueBlot® Anti-Mouse Ig Biotin  (ABIN1589967) Mouse TrueBlot® IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x...
Product cited in: Kong, Xu, Yu, Zhu, Andrews, Yoon, Kuo: "Regulation of Ca2+-induced permeability transition by Bcl-2 is antagonized by Drpl and hFis1." in: Molecular and cellular biochemistry, Vol. 272, Issue 1-2, pp. 187-99, 2005 (PubMed).

Zhang, Ozawa, Lee, Wen, Tan, Wadzinski, Seto: "Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4." in: Genes & development, Vol. 19, Issue 7, pp. 827-39, 2005 (PubMed).

DiPerna, Stack, Bowie, Boyd, Kotwal, Zhang, Arvikar, Latz, Fitzgerald, Marshall: "Poxvirus protein N1L targets the I-kappaB kinase complex, inhibits signaling to NF-kappaB by the tumor necrosis factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by toll-like receptors." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36570-8, 2004 (PubMed).

Lehtonen, Lehtonen, Kudlicka, Holthöfer, Farquhar: "Nephrin forms a complex with adherens junction proteins and CASK in podocytes and in Madin-Darby canine kidney cells expressing nephrin." in: The American journal of pathology, Vol. 165, Issue 3, pp. 923-36, 2004 (PubMed).

Tyagi, Agarwal, Harrison, Glode, Agarwal: "Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages." in: Carcinogenesis, Vol. 25, Issue 9, pp. 1711-20, 2004 (PubMed).