SAPAP Detection Set
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- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- Antibodies are supplied as affinity chromatography purified IgG.
- Components
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SAPAP1 Antibody, (50 μg)
SAPAP2 Antibody, (50 μg)
SAPAP3 Antibody, (50 μg)
SAPAP4 Antibody, (50 μg)
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- Application Notes
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These polyclonal antibodies can be used for detection of SAPAP1 - 4 by immunoblot at 0.5 - 2 μg/mL, and for detection of SAPAP1 - 3 by immunohistochemistry at 2.5 - 5 μg/mL, and Immunoflourescence.
Optimal dilutions for each application to be determined by the researcher. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Stable at 4 °C for three months, store at -20 °C for up to one year.
- Expiry Date
- 12 months
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- Background
- The members of the SAP90/PSD-95-associated protein (SAPAP) family (also known as the DLGAP family) specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1, -2 and -4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). SAPAP3 protein is targeted to dendrites. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. Multiple isoforms of the SAPAP proteins are known to exist.
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