Anti-DYKDDDDK Tag (L5) Affinity Gel

Details for Product No. ABIN2669061, Supplier: Log in to see
Target Name (Antigen)
Reactivity
Tag
Host
Rat
Clonality
Monoclonal
Application
Multiplex Assay (MA)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Characteristics The anti-DYKDDDDK tag affinity gel is composed of the L5 antibody conjugated to an agarose resin, which can be used for purification of proteins expressing the octapeptide, DYKDDDDK. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The binding capacity of the L5 agarose resin is greater than 0.8 mg/mL. The resin can be re-used at least five times (see technical bulletin for regeneration and storage). This affinity gel is also useful for immunoprecipitation of DYKDDDDK-tagged protein.
Purification The antibody was purified by affinity chromatography.
Components Anti-DYKDDDDK Tag (L5) Affinity Gel
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 50 % anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09 % Sodium azide. The volume specified for each catalog number indicates the volume of resin included.
Storage 4 °C
Storage Comment Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Expiry Date 12 months
Supplier Images
ELISA image for Anti-DYKDDDDK Tag (L5) Affinity Gel (ABIN2669061) Anti-DYKDDDDK Tag (L5) Affinity Gel
Western Blotting (WB) image for Anti-DYKDDDDK Tag (L5) Affinity Gel (ABIN2669061) Anti-DYKDDDDK Tag (L5) Affinity Gel (Image 2)
Background publications Grimley, Polyakova, Vamathevan, McKenary, Hayes, Patel, Smith, Bridges, Fosberry, Bhardwaja, Mouzon, Chung, Barrett, Richmond, Modha, Solari: "Over expression of wild type or a catalytically dead mutant of Sirtuin 6 does not influence NF?B responses." in: PLoS ONE, Vol. 7, Issue 7, pp. e39847, 2012 (PubMed).