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Mouse Naive/Memory T cell ID Panel

Catalog No. ABIN2669200
  • Reactivity
    Mouse
    Host
    Rat
    Clonality
    Monoclonal
    Application
    Flow Cytometry (FACS)
    Characteristics
    In mice, CD4+ T cells can be divided into naive and memory cells based on expression of adhesion molecules CD44 and CD62L. Naive T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naive and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.
    Components
    Mouse Naive/Memory T cell ID Panel, Rat IgG2b/Rat IgG2a/Rat IgG2a/Rat IgG2b
  • Application Notes
    This reagent contains one vial each of APC/Cy7 conjugated anti-CD3 (clone 17A2), PerCP/Cy5.5 conjugated anti-CD4 (clone RM4-5), APC conjugated anti-CD62L (clone MEL-14), and PE conjugated anti-CD44 (clone IM7) at optimal concentration for four-color flow cytometric analysis.
    Restrictions
    For Research Use only
  • Buffer
    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from prolonged exposure to light. Do not freeze.
    Storage
    4 °C
    Storage Comment
    The antibody solution should be stored undiluted between 2°C and 8°C.
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