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Mouse TNF ELISpot Set

ELISpot Reactivity: Mouse Colorimetric Cell Samples Uncoated
Catalog No. ABIN2691038
  • Target See all TNF alpha Kits
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Reactivity
    • 14
    • 12
    • 9
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Cell ELISA
    Application
    ELISpot
    Brand
    BD™ ELISPOT
    Sample Type
    Cell Samples
    Characteristics
    The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
    Components
    10 ELISPOT plates
    Unlabeled Capture Antibody
    Biotinylated Detection Antibody
    Enzyme Conjugate
    Certificate of Analysis
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  • Application Notes
    Unique strengths of the assay include the following:
    High sensitivity
    High throughput, high content analysis
    Minimal volume of biological material required
    Applicability to frozen/thawed biological samples
    Compatibility with other assays. For example, cells analyzed by BD™ ELISPOT can be transferred for cloning, proliferation assays,flow cytometry, or other methods of analysis.
    Comment

    BD™ ELISPOT Mouse TNF ELISPOT Set

    Plate
    Uncoated
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.
  • Helms, Boehm, Asaad, Trezza, Lehmann, Tary-Lehmann: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).

    Power, Grand, Ismail, Peters, Yurkowski, Bretscher: "A valid ELISPOT assay for enumeration of ex vivo, antigen-specific, IFNgamma-producing T cells." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 99-107, (1999) (PubMed).

    Tary-Lehmann, Hricik, Justice, Potter, Heeger: "Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure." in: Transplantation, Vol. 66, Issue 2, pp. 219-24, (1998) (PubMed).

    VanCott, Staats, Pascual, Roberts, Chatfield, Yamamoto, Coste, Carter, Kiyono, McGhee: "Regulation of mucosal and systemic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 4, pp. 1504-14, (1996) (PubMed).

    Fujihashi, McGhee, Beagley, McPherson, McPherson, Huang, Kiyono: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).

    Czerkinsky, Andersson, Ekre, Nilsson, Klareskog, Ouchterlony: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).

    Rönnblom, Cederblad, Sandberg, Alm: "Determination of herpes simplex virus-induced alpha interferon-secreting human blood leucocytes by a filter immuno-plaque assay." in: Scandinavian journal of immunology, Vol. 27, Issue 2, pp. 165-70, (1988) (PubMed).

    Czerkinsky, Nilsson, Nygren, Ouchterlony, Tarkowski: "A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 65, Issue 1-2, pp. 109-21, (1984) (PubMed).

    Sedgwick, Holt: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).

  • Target
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Alternative Name
    TNF (TNF alpha Products)
    Synonyms
    DIF Kit, TNF-alpha Kit, TNFA Kit, TNFSF2 Kit, RATTNF Kit, Tnfa Kit, tnf Kit, TNF-a Kit, TNFalpha Kit, Tnfsf1a Kit, TNFa Kit, cTNF Kit, Tnf-alpha Kit, tnfa-like Kit, TNF-ALPHA Kit, dif Kit, tnfa Kit, xtnf Kit, tnfsf2 Kit, tnf-alpha Kit, Cachectin Kit, tumor necrosis factor Kit, tumor necrosis factor b (TNF superfamily, member 2) Kit, tumor necrosis factor alpha Kit, tumor necrosis factor a (TNF superfamily, member 2) Kit, TNF Kit, Tnf Kit, tnf Kit, tnfb Kit, tnf-alpha Kit, LOC103694380 Kit, tnfa Kit
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