Z-VAD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412070

Additional Information

Immunogen: Sequence: Z-Val-Ala-Asp(OME)-FMK

Description: A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Molecular Weight: 468.0

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.

Purity: One spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: Reconstitution: Reconstitute in DMSO (20 mM)

Restrictions: For Research Use only

Publications

Product: Niikura, Dixit, Scott et al.: "BUB1 mediation of caspase-independent mitotic death determines cell fate." in: The Journal of cell biology, Vol. 178, Issue 2, pp. 283-96, 2007 (PubMed).

Upton, Austgen, Nishino et al.: "Caspase-2 cleavage of BID is a critical apoptotic signal downstream of endoplasmic reticulum stress." in: Molecular and cellular biology, Vol. 28, Issue 12, pp. 3943-51, 2008 (PubMed).

Liu, Enomoto, Lancto et al.: "Inhibition of apoptosis in Cryptosporidium parvum-infected intestinal epithelial cells is dependent on survivin." in: Infection and immunity, Vol. 76, Issue 8, pp. 3784-92, 2008 (PubMed).

Dai, Liu, Tang et al.: "Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7701-10, 2008 (PubMed).

Liu, Deng, Lancto et al.: "Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells." in: Infection and immunity, Vol. 77, Issue 2, pp. 837-49, 2009 (PubMed).

Ito, Yip, Katz et al.: "Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 969-83, 2009 (PubMed).

Ariyama, Kono, Matsuda et al.: "Decrease in membrane phospholipid unsaturation induces unfolded protein response." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22027-35, 2010 (PubMed).

Gao, Bauvy, Souquère et al.: "The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25570-81, 2010 (PubMed).

Fukazawa, Maeda, Matsuoka et al.: "Inhibition of Myc effectively targets KRAS mutation-positive lung cancer expressing high levels of Myc." in: Anticancer research, Vol. 30, Issue 10, pp. 4193-200, 2010 (PubMed).

Saha, Jiang, Mukai et al.: "RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses." in: Molecular cancer therapeutics, Vol. 9, Issue 11, pp. 3041-51, 2010 (PubMed).

Hara, Senga, Biswas et al.: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

Dolai, Pal, Yadav et al.: "Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13638-46, 2011 (PubMed).

Breinig, Mayer, Harjung et al.: "Heat shock protein 90-sheltered overexpression of insulin-like growth factor 1 receptor contributes to malignancy of thymic epithelial tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 8, pp. 2237-49, 2011 (PubMed).

Xie, Jiang, Wan et al.: "Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway." in: Journal of molecular cell biology, Vol. 3, Issue 4, pp. 250-9, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).