Z-YVAD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412073

Additional Information

Immunogen: Sequence: Z-Tyr-Val-Ala-Asp(OME)-FMK

Sequence: Z-Tyr-Val-Ala-Asp(OMe)-FMK

Format: Lyophilized

Description: A synthetic peptide that irreversibly inhibits activity of caspase-1. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).

Characteristics: Molecular formula: C₃₁H₃₉FN₄O₉

Molecular Weight: 630.7 Da

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.

Handling Advice: Protect from light and moisture

Purity: Single spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Shao: "Understanding the mechanisms of human tubal ectopic pregnancies: new evidence from knockout mouse models." in: Human reproduction (Oxford, England), Vol. 25, Issue 3, pp. 584-7, 2010 (PubMed).

Wei, Kadia, Tong et al.: "The combination of a histone deacetylase inhibitor with the Bcl-2 homology domain-3 mimetic GX15-070 has synergistic antileukemia activity by activating both apoptosis and autophagy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 15, pp. 3923-32, 2010 (PubMed).

Chang, Lin, Wu et al.: "Mechanisms underlying benzyl alcohol cytotoxicity (triamcinolone acetonide preservative) in human retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 52, Issue 7, pp. 4214-22, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

Lee, Hwang, Palaniyar et al.: "Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection." in: PLoS ONE, Vol. 7, Issue 4, pp. e35812, 2012 (PubMed).