Z-VDVAD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412074

Additional Information

Immunogen: Sequence: Z-Val-Asp(OMe)-Val-Ala-Asp(OME)-FMK

Description: A synthetic peptide that irreversibly inhibits activity of caspase-2. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Molecular Weight: 695.0

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.

Purity: One spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: Reconstitution: Reconstitute in DMSO (20 mM)

Restrictions: For Research Use only

Publications

Product: Upton, Austgen, Nishino et al.: "Caspase-2 cleavage of BID is a critical apoptotic signal downstream of endoplasmic reticulum stress." in: Molecular and cellular biology, Vol. 28, Issue 12, pp. 3943-51, 2008 (PubMed).

Höglund, Nilsson, Forshell et al.: "Myc sensitizes p53-deficient cancer cells to the DNA-damaging effects of the DNA methyltransferase inhibitor decitabine." in: Blood, Vol. 113, Issue 18, pp. 4281-8, 2009 (PubMed).

Nakajima, Nishimura, Nakaima et al.: "Cell type-dependent proapoptotic role of Bcl2L12 revealed by a mutation concomitant with the disruption of the juxtaposed Irf3 gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 30, pp. 12448-52, 2009 (PubMed).

Chang, Lin, Wu et al.: "Mechanisms underlying benzyl alcohol cytotoxicity (triamcinolone acetonide preservative) in human retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 52, Issue 7, pp. 4214-22, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).