Z-DEVD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412077

Additional Information

Immunogen: Sequence: Z-Asp(OMe)-Glu(OMe)-Val-Asp(OME)-FMK

Description: A synthetic peptide that irreversibly inhibits activity of DEVD-dependent caspases (e.g., caspase-3 and caspase-7) and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Molecular Weight: 668.0

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.

Purity: One spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: Reconstitution: Reconstitute in DMSO (20 mM)

Restrictions: For Research Use only

Publications

Product: Cunningham, Giuffrida, Roberts: "Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta." in: Endocrinology, Vol. 150, Issue 12, pp. 5539-48, 2009 (PubMed).

Du, Yamamoto, Ueda et al.: "Activated protein C rescues the retina from ischemia-induced cell death." in: Investigative ophthalmology & visual science, Vol. 52, Issue 2, pp. 987-93, 2011 (PubMed).

Abdul-Ghani, Dufort, Stiles et al.: "Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals." in: Molecular and cellular biology, Vol. 31, Issue 1, pp. 163-78, 2010 (PubMed).

Zhong, Carney, Boyan et al.: "17β-Estradiol regulates rat growth plate chondrocyte apoptosis through a mitochondrial pathway not involving nitric oxide or MAPKs." in: Endocrinology, Vol. 152, Issue 1, pp. 82-92, 2010 (PubMed).

Hara, Senga, Biswas et al.: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

Verma, Jain, Caseltine et al.: "TCR mimic monoclonal antibodies induce apoptosis of tumor cells via immune effector-independent mechanisms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 3265-76, 2011 (PubMed).

Vogt, Harmata, Coulombe et al.: "Causes and consequences of stolon regression in a colonial hydroid." in: The Journal of experimental biology, Vol. 214, Issue Pt 19, pp. 3197-205, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

Heinhuis, Koenders, van den Berg et al.: "Interleukin 32 (IL-32) contains a typical α-helix bundle structure that resembles focal adhesion targeting region of focal adhesion kinase-1." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5733-43, 2012 (PubMed).