Z-LEVD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412079

Additional Information

Immunogen: Sequence: Z-Leu-Glu(OMe)-Val-Asp(OME)-FMK

Sequence: Z-Leu-Glu(OMe)-Val-Asp(OMe)-FMK

Format: Lyophilized

Description: A synthetic peptide that irreversibly inhibits activity of LEVD-dependent caspases (e.g., caspase-4). The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).

Characteristics: Molecular formula: C₃₁H₄₅FN₄O₁₀

Molecular Weight: 652.7 Da

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.

Handling Advice: Protect from light and moisture

Purity: Single spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Akamatsu, Shibata, Ito et al.: "Riluzole induces apoptotic cell death in human prostate cancer cells via endoplasmic reticulum stress." in: Anticancer research, Vol. 29, Issue 6, pp. 2195-204, 2009 (PubMed).

Binet, Chiasson, Girard: "Interaction between arsenic trioxide (ATO) and human neutrophils." in: Human & experimental toxicology, Vol. 30, Issue 5, pp. 416-24, 2011 (PubMed).

Wei, Kadia, Tong et al.: "The combination of a histone deacetylase inhibitor with the Bcl-2 homology domain-3 mimetic GX15-070 has synergistic antileukemia activity by activating both apoptosis and autophagy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 15, pp. 3923-32, 2010 (PubMed).

Rosati, Sabatini, Rampino et al.: "Novel targets for endoplasmic reticulum stress-induced apoptosis in B-CLL." in: Blood, Vol. 116, Issue 15, pp. 2713-23, 2010 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).