Z-IETD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412083

Additional Information

Immunogen: Sequence: Z-ILe-Glu(OMe)-Thr-Asp(OME)-FMK

Description: A synthetic peptide that irreversibly inhibits activity of IETD-dependent caspases (e.g., caspase-8). The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Molecular Weight: 654.0

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary among different cells and culture conditions.

Purity: One spot by TLC

Buffer: Z-IETD-FMK in lyophilized powder

Storage: Reconstitution: Reconstitute in DMSO (20 mM)

Restrictions: For Research Use only

Publications

Product: Tang, Liu, Wang et al.: "Involvement of caspase 8 in apoptosis induced by ultrasound-activated hematoporphyrin in sarcoma 180 cells in vitro." in: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, Vol. 27, Issue 4, pp. 645-56, 2008 (PubMed).

Du, Yamamoto, Ueda et al.: "Activated protein C rescues the retina from ischemia-induced cell death." in: Investigative ophthalmology & visual science, Vol. 52, Issue 2, pp. 987-93, 2011 (PubMed).

Abdul-Ghani, Dufort, Stiles et al.: "Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals." in: Molecular and cellular biology, Vol. 31, Issue 1, pp. 163-78, 2010 (PubMed).

Saha, Jiang, Mukai et al.: "RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses." in: Molecular cancer therapeutics, Vol. 9, Issue 11, pp. 3041-51, 2010 (PubMed).

Hara, Senga, Biswas et al.: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).