Z-LEHD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412085

Additional Information

Immunogen: Sequence: Z-Leu-Glu(OMe)-His-Asp(OME)-FMK

Sequence: Z-Leu-Glu(OMe)-His-Asp(OMe)-FMK .TFA

Format: Lyophilized

Description: A synthetic peptide that irreversibly inhibits caspase-9 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).

Characteristics: Molecular formula: C₃₂H₄₃FN₆O₁₀.C₂F₃O₂H

Molecular Weight: 690.71 Da

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions. NOTE: For research purpose only! Not to be used in humans.

Handling Advice: Protect from light and moisture

Purity: Single spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Jin, Ojcius, Sun et al.: "Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways." in: Infection and immunity, Vol. 77, Issue 2, pp. 799-809, 2009 (PubMed).

Bian, Elner, Elner: "Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 50, Issue 12, pp. 6006-14, 2009 (PubMed).

Lipke, Matute-Bello, Herrero et al.: "Febrile-range hyperthermia augments lipopolysaccharide-induced lung injury by a mechanism of enhanced alveolar epithelial apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3801-13, 2010 (PubMed).

Du, Yamamoto, Ueda et al.: "Activated protein C rescues the retina from ischemia-induced cell death." in: Investigative ophthalmology & visual science, Vol. 52, Issue 2, pp. 987-93, 2011 (PubMed).

Hara, Senga, Biswas et al.: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

Verma, Jain, Caseltine et al.: "TCR mimic monoclonal antibodies induce apoptosis of tumor cells via immune effector-independent mechanisms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 3265-76, 2011 (PubMed).

Xie, Jiang, Wan et al.: "Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway." in: Journal of molecular cell biology, Vol. 3, Issue 4, pp. 250-9, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).