Z-ATAD-FMK

Application: Functional Studies (Func), Cell Culture (CC)

Catalog no.: ABIN412086

Additional Information

Immunogen: Sequence: Z-Ala-Thr-Ala-Asp(OME)-FMK

Description: A synthetic peptide that irreversibly inhibits activity of caspase-12. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Molecular Weight: 540.0

Application Details

Application Notes: We recommend using 2-10 μ M for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.

Purity: One spot by TLC

Buffer: 1 mg (or 5 mg) lyophilized powder

Storage: Reconstitution: Dissolve 1 mg of Z-ATAD-FMK with 92.5 µl pure DMSO to generate 20 mM Z-ATAD-FMK solution

Restrictions: For Research Use only

Publications

Product: Ou, Wu, Ip et al.: "Apoptosis induced by t10,c12-conjugated linoleic acid is mediated by an atypical endoplasmic reticulum stress response." in: Journal of lipid research, Vol. 49, Issue 5, pp. 985-94, 2008 (PubMed).

Fu, Minamino, Tsukamoto et al.: "Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition." in: Cardiovascular research, Vol. 79, Issue 4, pp. 600-10, 2008 (PubMed).

Trisciuoglio, Uranchimeg, Cardellina et al.: "Induction of apoptosis in human cancer cells by candidaspongiolide, a novel sponge polyketide." in: Journal of the National Cancer Institute, Vol. 100, Issue 17, pp. 1233-46, 2008 (PubMed).

Auner, Beham-Schmid, Dillon et al.: "The life span of short-lived plasma cells is partly determined by a block on activation of apoptotic caspases acting in combination with endoplasmic reticulum stress." in: Blood, Vol. 116, Issue 18, pp. 3445-55, 2010 (PubMed).

Dolai, Pal, Yadav et al.: "Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13638-46, 2011 (PubMed).

Suzuki, Lee, Matsuoka: "TDP-43-induced death is associated with altered regulation of BIM and Bcl-xL and attenuated by caspase-mediated TDP-43 cleavage." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13171-83, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi et al.: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).