Caspase-3 Substrate DEVD-AFC

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412342

Additional Information

Immunogen: Sequence: Ac-Asp-Glu-Val-Asp-AFC (AFC, 7-amino-4-trifluoromethyl coumarin)

Description: Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 μ M) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Molecular Weight: 729.0

Application Details

Protocol: 1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction. 2. Count cells and pellet 1-5 x 106 cells or use 50-200 μ g cell lysates if protein concentration has been measured. 3. Resuspend cells in 50 μ l of chilled Cell Lysis Buffer (Cat.# 1067-100). 4. Incubate cells on ice for 10 minutes. 5. Add 50 μ l of 2X Reaction Buffer (Cat.# 1068-20, -80) containing 10 mM DTT (Cat.# 1201-1) to each sample. 6. Add 5 μ l of the 1 mM DEVD-AFC (50 μ M final conc.) into each tube individually and incubate at 37 o C for 1-2 hour. 7. Read samples in a fluorometer equipped with a 400-nm excitation filter and 505-nm emission filter. For a plate-reading set-up, transfer the samples to a 96-well plate. You may perform the entire assay directly in a 96-well plate. 8. Fold-increase in caspase-3 activity can be determined by comparing these results with the level of the uninduced control.

Purity: >98% by HPLC analysis.

Restrictions: For Research Use only

Publications

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