Caspase-3 Inhibitor Z-DEVD-FMK

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412345

Additional Information

Immunogen: Sequence: Z-Asp-Glu-Val-Asp-FMK (FMK, fluoromethyl ketone)

Sequence: Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK

Format: Liquid

Description: A synthetic peptide inhibitor that irreversibly inhibits caspase-3 and related protease/caspase activity and blocks apoptosis. DEVD is the specific recognition sequence found in poly (ADP-ribose) polymerase (PARP). The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).

Characteristics: Molecular formula: C₃₀H₄₁FN₄O₁₂

Molecular Weight: 668.7 Da

Application Details

Application Notes: We recommend using 1000X dilutions for inhibiting caspase-3 activity in Jurkat cell culture (e.g., Add 1 μ l to 1 ml of culture medium). However, the optimal doses may vary for different cells and culture conditions.

Handling Advice: Protect from light and moisture

Purity: >98% by HPLC

Buffer: 100 μ l (2 mM) Z-DEVD-FMK in DMSO

Storage: -20°C

Expiry Date: 6-12 months

Restrictions: For Research Use only

Publications

Product: Yen, Mar, Craciunescu et al.: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

Song, Mahidhara, Zhou et al.: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).

Song, Zhou, Kim et al.: "Carbon monoxide promotes Fas/CD95-induced apoptosis in Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44327-34, 2004 (PubMed).

Wang, Grégori, Hullinger et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor (TNF) receptor 1/TNF-alpha expression." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).

Miura, Chen, Allen et al.: "A crucial role of caspase-3 in osteogenic differentiation of bone marrow stromal stem cells." in: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1704-13, 2004 (PubMed).

Kubota, Lee, Tsuchiya et al.: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005 (PubMed).

Bi, Stuelten, Kilts et al.: "Extracellular matrix proteoglycans control the fate of bone marrow stromal cells." in: The Journal of biological chemistry, Vol. 280, Issue 34, pp. 30481-9, 2005 (PubMed).

Gillet, Dewals, Farnir et al.: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Mao, Iwai, Keng et al.: "Norepinephrine-induced oxidative stress causes PC-12 cell apoptosis by both endoplasmic reticulum stress and mitochondrial intrinsic pathway: inhibition of phosphatidylinositol 3-kinase survival pathway." in: American journal of physiology. Cell physiology, Vol. 290, Issue 5, pp. C1373-84, 2006 (PubMed).

Lin, Cheung, Wu et al.: "15d-prostaglandin J2 protects brain from ischemia-reperfusion injury." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 3, pp. 481-7, 2006 (PubMed).

Kim, Zhang, Hitomi et al.: "Endoplasmic reticulum stress-induced caspase-4 activation mediates apoptosis and neurodegeneration in INCL." in: Human molecular genetics, Vol. 15, Issue 11, pp. 1826-34, 2006 (PubMed).

Nair, McNaught, González-Maeso et al.: "p53 mediates nontranscriptional cell death in dopaminergic cells in response to proteasome inhibition." in: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 39550-60, 2006 (PubMed).

Ma, Li, Chiu et al.: "Toll-like receptor 8 functions as a negative regulator of neurite outgrowth and inducer of neuronal apoptosis." in: The Journal of cell biology, Vol. 175, Issue 2, pp. 209-15, 2006 (PubMed).