Caspase-1 Inhibitor Z-YVAD-FMK

Details for Product No. ABIN412351
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Functional Studies (Func), Enzyme Activity Assay (EAA)
Pubmed 9 references available
Quantity 100 μL
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Immunogen Sequence: Z-Tyr-Val-Ala-Asp-FMK (FMK, fluoromethyl ketone)
Sequence Z-Tyr-Val-Ala-Asp(OMe)-FMK
Purity > 99 % by HPLC
Background A synthetic peptide that irreversibly inhibits ICE (interleukin-1beta converting enzyme, Ki = 0.76 nM) activity. YVAD is the preferred recognition sequence for ICE/caspase-1. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Molecular Weight 630.7 Da
Application Notes We recommend using 1000-5000X dilutions for inhibiting caspase-1 activity in Jurkat cell culture (e.g., Add 1 µL to 1-5 mL of culture medium). The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Liquid
Concentration 2 mM
Buffer DMSO
Handling Advice Protect from light and moisture.
Storage -20 °C
Expiry Date 6-12 months
Product cited in: Lhomme, Esser, Riva et al.: "Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages." in: Journal of lipid research, Vol. 54, Issue 11, pp. 2998-3008, 2013 (PubMed).

Shao: "Understanding the mechanisms of human tubal ectopic pregnancies: new evidence from knockout mouse models." in: Human reproduction (Oxford, England), Vol. 25, Issue 3, pp. 584-7, 2010 (PubMed).

Hewinson, Moore, Glover et al.: "A key role for redox signaling in rapid P2X7 receptor-induced IL-1 beta processing in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 12, pp. 8410-20, 2008 (PubMed).

Arai, Miyake, Voit et al.: "Death-effector domain-containing protein DEDD is an inhibitor of mitotic Cdk1/cyclin B1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2289-94, 2007 (PubMed).

Cruz, Rinna, Forman et al.: "ATP activates a reactive oxygen species-dependent oxidative stress response and secretion of proinflammatory cytokines in macrophages." in: The Journal of biological chemistry, Vol. 282, Issue 5, pp. 2871-9, 2007 (PubMed).

Klumpp, Rycyk, Chen et al.: "Uropathogenic Escherichia coli induces extrinsic and intrinsic cascades to initiate urothelial apoptosis." in: Infection and immunity, Vol. 74, Issue 9, pp. 5106-13, 2006 (PubMed).

Lin, Cheung, Wu et al.: "15d-prostaglandin J2 protects brain from ischemia-reperfusion injury." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 3, pp. 481-7, 2006 (PubMed).

Gillet, Dewals, Farnir et al.: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Yen, Mar, Craciunescu et al.: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

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Catalog No. ABIN412351
$ 165.00
Plus shipping costs $45.00
100 μL
$ 165.00

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