Caspase-1 Inhibitor Z-YVAD-FMK

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412352

Additional Information

Immunogen: Sequence: Z-Tyr-Val-Ala-Asp-FMK (FMK, fluoromethyl ketone)

Description: A synthetic peptide that irreversibly inhibits ICE (interleukin-1 β converting enzyme, Ki = 0.76 nM) activity. YVAD is the specific recognition sequence for ICE/caspase- 1. The inhibitor is designed as a methyl ester to facilitate cell permeability.

Molecular Weight: 630.0

Application Details

Application Notes: We recommend using 1000X dilutions for inhibiting caspase-3 activity in Jurkat cell culture (e.g., Add 1 μ l to 1 ml of culture medium). The optimal doses may vary for different cells and culture conditions.

Purity: >99% by HPLC analysis

Buffer: 20 μ l Z-YVAD-FMK (10 mM) in DMSO

Storage: Store at –20 o C. SHELF LIFE: 1 year under proper storage conditions

Restrictions: For Research Use only

Publications

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Arai, Miyake, Voit et al.: "Death-effector domain-containing protein DEDD is an inhibitor of mitotic Cdk1/cyclin B1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2289-94, 2007 (PubMed).

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Shao: "Understanding the mechanisms of human tubal ectopic pregnancies: new evidence from knockout mouse models." in: Human reproduction (Oxford, England), Vol. 25, Issue 3, pp. 584-7, 2010 (PubMed).