1X Annexin V Binding Buffer

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412353

Additional Information

Description: Buffer for use with the BioVision’s multi-color bulk annexin V reagents including annexin V- FITC (Cat# 1001-1000), annexin V-EGFP (Cat.# 1004-1000), annexin V-Cy3 (Cat.# 1002- 1000), annexin V-Cy5 (1013-1000), annexin V-Biotin (Cat.# 1003-100), annexin V-PE (1014- 1000) and unlabeled annexin V (1005-100). During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca 2+ -dependent affinity for PS and therefore serves as a probe for detecting apoptosis. BioVision’s 1X Annexin V Binding Buffer is ready to use for annexin V binding assay to detect PS exposure during apoptosis. For a complete annexin V binding assay protocol, please refer to Annexin V Apoptosis Detection Kits: Annexin V-FITC Apoptosis Detection Kit (Cat.#: K101-25, -100, -400), Annexin V-EGFP Apoptosis Detection Kit (K104-25, -100, -400), Annexin V-Cy3 Apoptosis Detection Kit (K102-25, -100, -400), Annexin V-Cy5 Apoptosis Detection Kit (K103-25, -100, -400), Annexin V-PE Apoptosis Detection Kit (K128-25, -100, -400), Annexin V-Biotin Apoptosis Detection Kit (K109-25, -100, -400).

Application Details

Restrictions: For Research Use only

Publications

Janda, Visalli, Colica et al.: "The protective effect of tianeptine on Gp120-induced apoptosis in astroglial cells: role of GS and NOS, and NF-κB suppression." in: British journal of pharmacology, 2010 (PubMed).

Product: Xi, Dyer, Kimak et al.: "Decreased STAT1 expression by promoter methylation in squamous cell carcinogenesis." in: Journal of the National Cancer Institute, Vol. 98, Issue 3, pp. 181-9, 2006 (PubMed).

Guida, Bisso, Fenollar-Ferrer et al.: "Peptide aptamers targeting mutant p53 induce apoptosis in tumor cells." in: Cancer research, Vol. 68, Issue 16, pp. 6550-8, 2008 (PubMed).

Kim, Shu, Xu et al.: "Chemical biology investigation of cell death pathways activated by endoplasmic reticulum stress reveals cytoprotective modulators of ASK1." in: The Journal of biological chemistry, Vol. 284, Issue 3, pp. 1593-603, 2009 (PubMed).

Henderson, Shaw, Wang et al.: "UA62784, a novel inhibitor of centromere protein E kinesin-like protein." in: Molecular cancer therapeutics, Vol. 8, Issue 1, pp. 36-44, 2009 (PubMed).

Mirza, Teo, Upcroft et al.: "A rapid, high-throughput viability assay for Blastocystis spp. reveals metronidazole resistance and extensive subtype-dependent variations in drug susceptibilities." in: Antimicrobial agents and chemotherapy, Vol. 55, Issue 2, pp. 637-48, 2011 (PubMed).