Caspase-8 Inhibitor Z-IETD-FMK

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412376

Additional Information

Immunogen: Sequence: Z-Ile-Glu-Thr-Asp-FMK (FMK, fluoromethyl ketone)

Sequence: Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK

Format: Liquid

Description: A synthetic peptide that irreversibly inhibits FLICE and related protease/caspase activity and blocks apoptosis. IETD is the specific recognition sequence found in CPP32 proenzyme. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups.)

Characteristics: Molecular formula: C₃₀H₄₃FN₄O₁₁

Molecular Weight: 654.7 Da

Application Details

Application Notes: We recommend using 1000X dilutions for inhibiting caspase-8 activity in Jurkat cell culture (e.g., Add 1 μ l to 1 ml of culture medium). The optimal doses may vary for different cells and culture conditions.

Handling Advice: Protect from light and moisture

Purity: >99% by HPLC

Buffer: 100 μ l Z-IETD-FMK (2 mM) in DMSO

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Frost, Delikat, Al-Mehairi et al.: "Regulation of p27KIP1 in Epstein-Barr virus-immortalized lymphoblastoid cell lines involves non-apoptotic caspase cleavage." in: The Journal of general virology, Vol. 82, Issue Pt 12, pp. 3057-66, 2001 (PubMed).

Yen, Mar, Craciunescu et al.: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

Song, Mahidhara, Zhou et al.: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).

Song, Zhou, Kim et al.: "Carbon monoxide promotes Fas/CD95-induced apoptosis in Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44327-34, 2004 (PubMed).

Wang, Grégori, Hullinger et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor (TNF) receptor 1/TNF-alpha expression." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).

Gillet, Dewals, Farnir et al.: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Deng, Yin, Lu et al.: "The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 91, Issue 1, pp. 59-69, 2006 (PubMed).

Teachey, Obzut, Axsom et al.: "Rapamycin improves lymphoproliferative disease in murine autoimmune lymphoproliferative syndrome (ALPS)." in: Blood, Vol. 108, Issue 6, pp. 1965-71, 2006 (PubMed).

Tang, Liu, Wang et al.: "Involvement of caspase 8 in apoptosis induced by ultrasound-activated hematoporphyrin in sarcoma 180 cells in vitro." in: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, Vol. 27, Issue 4, pp. 645-56, 2008 (PubMed).

Westphal, Sytnyk, Schachner et al.: "Clustering of the neural cell adhesion molecule (NCAM) at the neuronal cell surface induces caspase-8- and -3-dependent changes of the spectrin meshwork required for NCAM-mediated neurite outgrowth." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 42046-57, 2010 (PubMed).

Chuang, Lin, Tai et al.: "Preferential involvement of mitochondria in Toll-like receptor 3 agonist-induced neuroblastoma cell apoptosis, but not in inhibition of cell growth." in: Apoptosis : an international journal on programmed cell death, 2011 (PubMed).