Caspase-9 Inhibitor Z-LEHD-FMK

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412393

Additional Information

Immunogen: Sequence: Z-Leu-Glu-His-Asp-FMK (FMK, fluoromethyl ketone)

Sequence: Z-Leu-Glu(OMe)-His-Asp(OMe)-FMK

Format: Liquid

Description: A synthetic peptide that irreversibly inhibits caspase-9 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).

Characteristics: Molecular formula: C₃₂H₄₃FN₆O₁₀

Molecular Weight: 690.7 Da

Application Details

Application Notes: Centrifuge the vial prior to opening. We recommend using 1000-5000X dilutions for inhibiting caspase-9 activity in cell culture (e.g., Add 1 μ l to 1-5 ml of culture medium). The optimal doses may vary for different cells and culture conditions.

Handling Advice: Protect from light and moisture

Purity: >98% by HPLC

Buffer: 20 μ l (10 mM) Z-LEHD-FMK in DMSO

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Chatterjee, Brite, Matsumura: "Induction of apoptosis of integrin-expressing human prostate cancer cells by cyclic Arg-Gly-Asp peptides." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 10, pp. 3006-11, 2001 (PubMed).

Morishima, Nakanishi, Takenouchi et al.: "An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34287-94, 2002 (PubMed).

Yen, Mar, Craciunescu et al.: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

Beaupre, Cepero, Obeng et al.: "R115777 induces Ras-independent apoptosis of myeloma cells via multiple intrinsic pathways." in: Molecular cancer therapeutics, Vol. 3, Issue 2, pp. 179-86, 2004 (PubMed).

Song, Zhou, Kim et al.: "Carbon monoxide promotes Fas/CD95-induced apoptosis in Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44327-34, 2004 (PubMed).

Wang, Grégori, Hullinger et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor (TNF) receptor 1/TNF-alpha expression." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).

Yao, Nguyen, Pike: "Beta-amyloid-induced neuronal apoptosis involves c-Jun N-terminal kinase-dependent downregulation of Bcl-w." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 5, pp. 1149-58, 2005 (PubMed).

Gillet, Dewals, Farnir et al.: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Deng, Yin, Lu et al.: "The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 91, Issue 1, pp. 59-69, 2006 (PubMed).

Teachey, Obzut, Axsom et al.: "Rapamycin improves lymphoproliferative disease in murine autoimmune lymphoproliferative syndrome (ALPS)." in: Blood, Vol. 108, Issue 6, pp. 1965-71, 2006 (PubMed).

Jin, Ojcius, Sun et al.: "Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways." in: Infection and immunity, Vol. 77, Issue 2, pp. 799-809, 2009 (PubMed).

Ying, Yang, Tsai et al.: "Fisetin induces apoptosis in human cervical cancer HeLa cells through ERK1/2-mediated activation of caspase-8-/caspase-3-dependent pathway." in: Archives of toxicology, 2011 (PubMed).