Caspase-12 Inhibitor

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412401

Additional Information

Immunogen: Sequence: Z-Ala-Thr-Ala-Asp(OMe)-FMK (FMK, fluoromethyl ketone)

Description: A synthetic peptide that irreversibly inhibits caspase-12 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability.

Molecular Weight: 540

Application Details

Application Notes: We recommend using up to 1000-5000X dilutions for inhibiting caspase-12 activity in cell culture (e.g., Add 1 μ l to 1 ml of culture medium). The optimal doses may vary for different cells and culture conditions.

Purity: >95% by HPLC analysis.

Buffer: 20 μ l (10 mM) Z-ATAD-FMK in DMSO

Storage: Store at –20 o C. SHELF LIFE: 6-12 months under proper storage conditions

Restrictions: For Research Use only

Publications

Product: Ou, Wu, Ip et al.: "Apoptosis induced by t10,c12-conjugated linoleic acid is mediated by an atypical endoplasmic reticulum stress response." in: Journal of lipid research, Vol. 49, Issue 5, pp. 985-94, 2008 (PubMed).

Fu, Minamino, Tsukamoto et al.: "Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition." in: Cardiovascular research, Vol. 79, Issue 4, pp. 600-10, 2008 (PubMed).

Trisciuoglio, Uranchimeg, Cardellina et al.: "Induction of apoptosis in human cancer cells by candidaspongiolide, a novel sponge polyketide." in: Journal of the National Cancer Institute, Vol. 100, Issue 17, pp. 1233-46, 2008 (PubMed).

Bian, Elner, Elner: "Regulated expression of caspase-12 gene in human retinal pigment epithelial cells suggests its immunomodulating role." in: Investigative ophthalmology & visual science, Vol. 49, Issue 12, pp. 5593-601, 2008 (PubMed).

Zeng, Li, He et al.: "Adaptive basal phosphorylation of eIF2α is responsible for resistance to cellular stress-induced cell death in Pten-null hepatocytes." in: Molecular cancer research : MCR, Vol. 9, Issue 12, pp. 1708-17, 2011 (PubMed).