Caspase-5 Inhibitor Z-WEHD-FMK

Application: Functional Studies (Func), Enzyme Activity Assay (EAA)

Catalog no.: ABIN412403

Additional Information

Immunogen: Sequence: Z-Trp-Glu-His-Asp-FMK (FMK, fluoromethyl ketone)

Description: A synthetic peptide that irreversibly inhibits caspase-5 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability.

Molecular Weight: 877.0

Application Details

Application Notes: We recommend using 1000-5000X dilutions for inhibiting caspase-5 activity in Jurkat cell culture (e.g., Add 1 μ l to 1-5 ml of culture medium). The optimal doses may vary for different cells and culture conditions.

Purity: >99% by HPLC analysis.

Buffer: 20 μ l Z-WEHD-FMK (10 mM) in DMSO

Storage: Store at –20 o C. SHELF LIFE: 1 year under proper storage conditions

Restrictions: For Research Use only

Publications

Product: Coletti, Yang, Marazzi et al.: "TNFalpha inhibits skeletal myogenesis through a PW1-dependent pathway by recruitment of caspase pathways." in: The EMBO journal, Vol. 21, Issue 4, pp. 631-42, 2002 (PubMed).

Gillet, Dewals, Farnir et al.: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).