ECL substrate (Mark-It Glow liquid)

Application: Western Blotting (WB)

Catalog no.: ABIN457495

Additional Information

Format: Liquid

Description: The Mark-It Glow product line represents a series of novel very sensitive two component substrates for the development of Western-Blots based on the enhanced luminol technique catalysed by horseradish peroxidase (HRP) conjugated antibodies/secondary reagents. Mark-It Glow is available as standard formulation Mark-It Glow Liquid, which is suitable for the development of any standard Western Blot with a sensitivity in the picomolar range. In addition, with the Mark-It Glow Ultra formulation we offer a second system with an extremely sensitive detection limit enabling the detection of even low abundant cellular proteins in the femtomolar region. Unlike other substrates, you don't have to mix Mark-It Glow. You simply position your blot on a plastic sheet and spray as much as you need from bottle 1 and then from bottle 2. Besides the ease of use the performance of the reagent is excellent. For users with very high consumption we also provide a maxi-pack of Mark-It Glow Liquid without sprayhead.

Application Details

Application Notes: 500 mL are sufficient for 25.000 cm2 (ca. 250 mini-gels)

Components: 250 ml Solution A + 250 ml Solution B in capped flasks (transparent and brown PP)

Storage: Store at 2 to 8°C

Restrictions: For Research Use only

Images

Western Blot developed with Mark-It Glow Liquid ECL substrate. Decreasing amounts of a control protein were subjected to Western Blotting (Lanes 1-7: 300ng, 100 ng, 30 ng, 10 ng, 3 ng 1 ng, 300 pg). The blocked membrane was probed with a specific primary antibody and bound antibodies were detected with a specific HRP conjugate. Visualization was performed using MicroMol’s Mark-It Glow Liquid ECL substrate.

ECL substrate Mark-It Glow Liquid is superior to its competitors on the market. Mark-It Glow Liquid as well as one of the leading ECL substrates on the market were activated by horseradish peroxidase (0.05 nU/ml) and light emission was measured luminometric at the indicated points of time.