|
Characteristics
|
For the immunoprecipitation of RFP-fusion proteins from cellular extracts.
|
|
Description
|
Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these RFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via immunoprecipitation using the RFP-Trap. The RFPTrap_A enables purification of any protein of interest fused to RFP.
|
|
Specificity
|
RFP-Trap efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby and RFP-tagged fusion proteins.
|
