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RFP-Trap (coupled to magnetic particles)
| Antigen | Red Fluorescent Protein (RFP) |
| Application |
Immunoprecipitation (IP)
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1 reference available |
| Catalog no. | ABIN509412 |
| Quantity | 20 reactions (500 µl resin) |
| Price | 429.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
Additional Information
| Characteristics | For the immunoprecipitation of RFP-fusion proteins from cellular extracts. |
| Description | Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these RFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via immunoprecipitation using the RFP-Trap. The RFPTrap_A enables purification of any protein of interest fused to RFP. |
| Specificity | RFP-Trap efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby and RFP-tagged fusion proteins. |
Application Details
| Components | RFP-Trap coupled to magnetic particles, particle size 0.5 - 1 µm (particles from chemicell), stored in 1x PBS, 0.1% BSA |
| Storage | Store material at 4°C, do not freeze |
| Research Area | Tags/Labels |
| Restrictions | For Research Use only |
Publications
| Publications |
Lefebvre, Klaus-Heisen, Pietraszewska-Bogiel et al.: "Role of N-glycosylation sites and CXC motifs in trafficking of Medicago trunculata Nod Factor Perception protein to the plasma membrane." in: The Journal of biological chemistry, 2012 (PubMed).
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