GFP-Booster_Atto488

Antigen: Green Fluorescent Protein (GFP)

Application: Immunofluorescence (IF)

Catalog no.: ABIN509419

Additional Information

Description: GFP is a powerful tool to study protein localization and dynamics in living cells. However, the photo stability and the quantum efficiency of GFP is not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples from cells expressing GFP-fusion proteins to visualize specific structures. Furthermore, many cell biological methods such as HCl treatment for BrdU-detection, the EdU-Click-iT™ treatment or heat denaturation for FISH lead to disruption of GFP signal. Here we offer our GFP-Booster _Atto488, a specific GFP-binding protein coupled to the fluorescent dye ATTO 488 (from ATTO-TEC) for reactivation, boosting and stabilization of GFP.

Application Details

Components: GFP-Trap® coupled to fluorescent dye ATTO 488

Concentration: 1 mg/ml

Storage: Shipped at RT. Store at 4°C, do not freeze. Protect from light.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Enhancement of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click-iT™ treament leads to disruption of GFP signal. GFP-Booster labels GFP fusion proteins and thus reactivates and boosts the fluorescence.