20S Proteasome Activation Solution (100X)

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Application
Activation (Act)
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Purpose Activation of 20S Proteasome
Characteristics The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator alpha.
Application Notes Dilute to 1X for activation for a final concentration of 0.03 % in assay. Dilute in reaction buffers prior to adding proteasome (do not add directly to solutions already containing proteasome). Use with peptide substrates containing arginine residues is not recommended, as precipitation of the substrate may occur. In these instances non-chemical activation may provide better results.
Comment

This buffer contains the optimal formulation for the activation of all isoforms of constitutive 20S and immuno 20S proteasome enzymes including human, rabbit, rat, and mouse.

Restrictions For Research Use only
Format Liquid
Buffer 3 % SDS.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months