Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody

Antigen: Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2)

Synonyms: GMCSF, MGC131935, MGC138897, Csfgm, Gm-CSf, MGI-IGM, MGC151255, MGC151257, Gmcsf, Gm-csf, CSF2, GM-CSF

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN100060

Additional Information

Alternative name: Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF)

Immunogen: This whole rabbit serum dy was prepared by repeated immunizations with recombinant human GM-CSF produced in Chinese Hamster cells.

Format: Liquid (sterile filtered)

Specificity: This antiserum has been heated to 56oC for 30 minutes. In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. Endotoxin Content: <10 pg/ ul by LAL method.

Application Details

Application Notes: Note: This antiserum against Human GM-CSF has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry and immunoblotting. Reactivity in other immunoassays is unknown. Recommended Dilutions : This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code ABIN101990) and TMB as a substrate. A working dilution of 1:400 is suggested for this application. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by ELISA against GM-CSF using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code ABIN101990) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:600 to 1:1,200 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay. Optimal titers for other applications should be determined by the researcher. See below for use in neutralizations.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 None.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening.

Research Area: Cytokines

Restrictions: For Research Use only