Granulocytes antibody (Biotin)

Antigen: Granulocytes

Clonality: Monoclonal (RB6-8C5)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Catalog no.: ABIN100072

Additional Information

Isotype: IgG2b

Clone: RB6-8C5

Specificity: Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes Mouse Granulocytes . This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution: The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2

Application Details

Application Notes: IHC (Frozen), FC, IP, WB Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 1.0 g/ml. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Granulocytes . A suggested method would be to stain cells expressing Granulocytes with Anti-Mouse Granulocytes at =1.0 g per 1.0 X 106 cells in a 100 l total staining volume, followed by UltraAvidin- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104). Each Investigator should determine their own optimal working dilution for specific applications.

Concentration: 0.5 mg/ml

Buffer: This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Storage: This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only