Granulocytes antibody (FITC)

Antigen: Granulocytes

Clonality: Monoclonal (RB6-8C5)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Catalog no.: ABIN100073

Additional Information

Isotype: IgG2b

Clone: RB6-8C5

Specificity: Rat Anti-Mouse Granulocytes recognizes Mouse Granulocytes . This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution: The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2

Application Details

Application Notes: IHC (Frozen), FC, IP, WB Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 1.0 g/ml. FC: This antibody can be used at a concentration of = 1.0 g per 100 l of whole blood (or per 1.0 x 10^6 cells in a 100 l total staining volume). Each Investigator should determine their own optimal working dilution for specific applications.

Concentration: 0.5 mg/ml

Buffer: This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Storage: This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only